Development and evaluation of a rapid recombinase polymerase amplification assay for the detection of human enterovirus 71

Archives of Virology
Dan YinGuifang Hu

Abstract

Enterovirus 71 (EV71) is one of the most common pathogens of hand, foot, and mouth disease (HFMD). A rapid reverse transcription recombinase polymerase amplification (RT-RPA) assay was established to detect EV71 subgenotype C4 (EV71-C4). The 95% detection limit of the RT-RPA was 3.767 log10 genomic copies (LGC)/reaction. The specificity was 100%. In a clinical sample evaluation, this approach demonstrated sufficient clinical performance when compared with a commercial RT-qPCR diagnostic kit. Thus, the RT-RPA assay may be a promising alternative for the detection of EV71-C4.

References

Mar 18, 2000·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·B A BrownM A Pallansch
Feb 29, 2008·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Eng Lee TanChit Laa Poh
Mar 10, 2009·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Yong ZhangWen-Bo Xu
Oct 23, 2010·Lancet Neurology·Mong How OoiTom Solomon
Feb 4, 2014·The Lancet Infectious Diseases·Weijia XingHongjie Yu
Jan 9, 2015·Journal of Clinical Microbiology·Boon-Teong TeohSazaly AbuBakar
Nov 13, 2015·Euro Surveillance : Bulletin Européen Sur Les Maladies Transmissibles = European Communicable Disease Bulletin·Oumar FayeAmadou Alpha Sall
Feb 9, 2017·Clinical and Experimental Vaccine Research·Eun-Je YiSun-Young Chang
Dec 21, 2017·Euro Surveillance : Bulletin Européen Sur Les Maladies Transmissibles = European Communicable Disease Bulletin·Bingyi YangHongjie Yu

❮ Previous
Next ❯

Datasets Mentioned

BETA
KX272843.1
KU888146.1
KJ188641.1
KM023191.1
KJ188632.1
GQ487689.1
GQ487685.1
GQ487669.1
JX678878.1
KF444809.1

Methods Mentioned

BETA
PCR
in vitro transcription

Software Mentioned

MEGA
MEDCALC
SPSS

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.