PMID: 11931037Apr 5, 2002Paper

Development and evaluation of a species diagnostic polymerase chain reaction-restriction fragment-length polymorphism procedure for cryptic members of the Culex sitiens (Diptera: Culicidae) subgroup in Australia and the southwest Pacific

Journal of Medical Entomology
N W BeebeR D Cooper

Abstract

Members of the Culex sitiens subgroup are important vectors of arboviruses, including Japanese encephalitis virus, Murray Valley encephalitis virus and Ross River virus. Of the eight described species, Cx. annulirostris Skuse, Cx. sitiens Wiedemann, and Cx. palpalis Taylor appear to be the most abundant and widespread throughout northern Australia and Papua New Guinea (PNG). Recent investigations using allozymes have shown this subgroup to contain cryptic species that possess overlapping adult morphology. We report the development of a polymerase chain reaction-restriction fragment-length polymorphism (PCR-RFLP) procedure that reliably separates these three species. This procedure utilizes the sequence variation in the ribosomal DNA ITS1 and demonstrates species-specific PCR-RFLP profiles from both colony and field collected material. Assessment of the consistency of this procedure was undertaken on mosquitoes sampled from a wide geographic area including Australia, PNG, and the Solomon Islands. Overlapping adult morphology was observed for Cx. annulirostris and Cx. palpalis in both northern Queensland and PNG and for all three species at one site in northwest Queensland.

References

Jan 1, 1994·Archives of Virology·J S MackenzieD W Smith
Oct 1, 1993·The American Journal of Tropical Medicine and Hygiene·J A ScottF H Collins
Feb 1, 1996·Insect Molecular Biology·F H Collins, S M Paskewitz
Jan 1, 1997·The American Journal of Tropical Medicine and Hygiene·S A RitchieA van den Hurk
Feb 17, 2000·International Journal for Parasitology·N W Beebe, R D Cooper

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Citations

Dec 15, 2010·Annals of Tropical Medicine and Parasitology·A F van den HurkC C Jansen
Jul 15, 2009·Acta Tropica·Arif-Ul- HasanYoshiyuki Kakehi
May 2, 2006·Journal of the American Mosquito Control Association·Andrew E van den HurkScott A Ritchie
Mar 23, 2018·Parasitology·Nigel W Beebe
Jul 26, 2018·Practical Neurology·Tehmina Bharucha, Hadi Manji
Feb 5, 2008·Journal of the American Mosquito Control Association·Ilia RochlinScott R Campbell
Apr 13, 2012·Journal of Medical Entomology·Sonja Hall-MendelinAndrew F Van den Hurk
May 10, 2017·Infectious Diseases of Poverty·Yuan FangYi Zhang

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