PMID: 9530608Apr 8, 1998Paper

Development and evaluation of an ELISA to measure antibody responses to both the nucleocapsid and spike proteins of canine coronavirus

Journal of Immunoassay
M L Palmer-DensmoreM I Sabara

Abstract

A rapid and reproducible enzyme linked immunosorbent assay (ELISA) was developed for detection of canine coronavirus (CCV) specific antibodies directed to both the nucleocapsid (NC) and the spike (S) proteins. The coating antigen, a methanol-treated, S-protein enriched preparation, was produced by subjecting infected cells to Triton X-114 detergent followed by phase separation. The sensitivity of this assay was determined by following the course of infection in dogs experimentally infected with CCV. The specificity of the antibody response was determined by Western blot analysis and supported the increased magnitude of the ELISA response and the presence of serum neutralizing (SN) antibody. Due to the sensitivity and specificity of the IgG response detected by this assay it can be used to determine both virus exposure and vaccine efficacy.

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Mar 25, 1975·Biochimica Et Biophysica Acta·A Helenius, K Simons
Jul 1, 1991·Research in Veterinary Science·B J TennantC J Gaskell
Jan 1, 1990·Advances in Experimental Medicine and Biology·H VennemaW J Spaan
Jun 1, 1987·Nihon juigaku zasshi. The Japanese journal of veterinary science·K TuchiyaS Konishi
Feb 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·P A Maher, S J Singer
Mar 1, 1981·The Journal of General Virology·D Cavanagh
Jan 1, 1981·Archives of Virology·H J Hasony, M R Macnaughton
Jun 1, 1994·Zentralblatt für Veterinärmedizin. Reihe B. Journal of veterinary medicine. Series B·M Martin-CalvoI Simarro
Jan 1, 1994·Methods in Enzymology·J S Brusca, J D Radolf
Feb 1, 1987·Planta medica·H H AppelV Gambaro
Jul 1, 1980·The Veterinary Quarterly·J VandenbergheJ Hoorens

❮ Previous
Next ❯

Citations

Mar 28, 2006·Trends in Biotechnology·Alexandra Adams, Kim D Thompson

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antibody Specificity

Antibodies produced by B cells are highly specific for antigen as a result of random gene recombination and somatic hypermutation and affinity maturation. As the main effector of the humoral immune system, antibodies can neutralize foreign cells. Find the latest research on antibody specificity here.

Related Papers

Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases
A YüceE S Uçan
International Immunopharmacology
Ruth Arnon, Tamar Ben-Yedidia
© 2022 Meta ULC. All rights reserved