PMID: 11899028Mar 20, 2002Paper

Development and evaluation of an enzyme-linked immunosorbent assay for the serodiagnosis of pythiosis in dogs

Journal of Veterinary Internal Medicine
A M GrootersKathy L O'Reilly

Abstract

Pythiosis (caused by the aquatic oomycete Pythium insidiosum) is a devastating and often fatal cause of either severe transmural gastroenteritis or locally invasive subcutaneous disease in dogs living in the southeastern United States. Although early diagnosis is essential for successful treatment, tools available for this task are limited. Therefore, we developed and evaluated an enzyme-linked immunosorbent assay (ELISA) for the detection of anti-P insidiosum antibodies in canine serum. A soluble mycelial extract of P insidiosum was utilized as antigen in the ELISA, which was used to evaluate serum from 43 dogs with pythiosis, 8 dogs with lagenidiosis (another canine oomycosis), 16 dogs with nonoomycotic fungal or algal infections, 22 dogs with nonfungal gastrointestinal or skin disease, and 55 healthy dogs. Results were expressed as percent positivity (PP) relative to a strong positive control serum run on each plate. Medians and ranges for each of the 5 groups were as follows: pythiosis (81.7%, 50.6-98.5%), lagenidiosis (17.3%, 11.3-29.2%), other fungal or algal infections (8.2%, 4.7-15.4%), nonfungal gastrointestinal or skin disease (6.2%, 3.9-20.7%), and healthy dogs (6.7%, 3.0-15.2%). When using a cutoff value of 40% PP, ...Continue Reading

Citations

Sep 17, 2002·Veterinary Dermatology·Nadine R ZnajdaRosanna Marsella
Dec 3, 2005·Medical Mycology·Leonel Mendoza, Joseph C Newton
Jul 24, 2003·Journal of the American Veterinary Medical Association·Patrick HenselLeonel Mendoza
Oct 18, 2011·Journal of the American Veterinary Medical Association·Kelley M ThiemanNicholas J Bacon
Jul 21, 2012·Journal of the American Veterinary Medical Association·Chad W SchmiedtKaren K Cornell
Jun 9, 2004·Journal of Clinical Microbiology·Andrew M SchurkoGlen R Klassen

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