Development and evaluation of an in vitro model for the analysis of cigarette smoke effects on cultured cells and tissues

Journal of Pharmacological and Toxicological Methods
David BernhardG Wick

Abstract

Smokers have an increased risk for a variety of diseases. Among the most prominent is atherosclerosis, the leading cause of death in the Western world. Although this conjunction is accepted knowledge, the basic biological mechanisms and the identities of the active tobacco smoke constituents surprisingly are still unknown. One reason for this is the lack of accurate in vitro models. Cell culture experiments, including cell morphology and cell death analyses, high-performance liquid chromatography, and liquid chromatography coupled to mass spectrometry via an electrospray ionization interface allowing collision-induced dissociation analyses, were applied. In this study, we present and validate an in vitro model that has proven to be useful for standardized studies of cellular and histological effects of cigarette smoke. The system consists of a cigarette smoke sampling device in which water-soluble cigarette smoke constituents pass over from the gas phase into the aqueous phase resulting in nicotine concentrations identical to the in vivo concentrations, suggesting in vivo similar conditions for gas-to-liquid compound exchange.

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Citations

Feb 12, 2014·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Matthew R CostelloFrances M Leslie
Dec 29, 2010·Cell Stress & Chaperones·Krisztina GálVeronika Müller
Aug 19, 2011·International Journal of Biological Sciences·Zhao-Li ChenJun-Wen Li
Mar 15, 2008·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Carla M T BauerKaren L Mossman
Jul 30, 2011·Journal of Molecular and Cellular Cardiology·Simone Barbara KreutmayerDavid Bernhard
Jun 21, 2011·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Andrew E HoganDonal O'Shea
Dec 7, 2010·Immunobiology·Hartwig P HuemerMarion Pavlic
Sep 1, 2009·Environmental Toxicology and Pharmacology·Lou JianlinHe Jiliang
Apr 15, 2008·Atherosclerosis·Blair HendersonGeorg Wick
Dec 5, 2006·Atherosclerosis·Astrid SchmiederDavid Bernhard
Aug 30, 2005·Annals of the New York Academy of Sciences·M KnoflachG Wick
Sep 25, 2012·Experimental and Toxicologic Pathology : Official Journal of the Gesellschaft Für Toxikologische Pathologie·Hidenori NaraMichaela Aufderheide
Dec 14, 2011·PloS One·Beata KosmiderRobert J Mason
May 5, 2017·Inhalation Toxicology·Laura J LeslieLindsay J Marshall
Apr 18, 2018·Journal of Applied Toxicology : JAT·Michał OtrębaAndrzej Sobczak
Dec 7, 2007·Journal of Leukocyte Biology·M Firoz MianAli A Ashkar
Jan 28, 2006·Arteriosclerosis, Thrombosis, and Vascular Biology·David BernhardGeorg Wick
Jan 6, 2006·IUBMB Life·David BernhardGeorg Wick
Oct 19, 2006·The Journal of Clinical Endocrinology and Metabolism·T J CawoodD O'Shea
Feb 12, 2008·Proceedings of the National Academy of Sciences of the United States of America·Climent Casals-PascualCharles R J C Newton
Aug 12, 2014·Pediatric and Developmental Pathology : the Official Journal of the Society for Pediatric Pathology and the Paediatric Pathology Society·Manuel NistalMiguel Reyes-Múgica
Jun 30, 2005·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·David BernhardGeorg Wick
May 20, 2020·Frontiers in Molecular Biosciences·Yaoyun DuanKunfu Ouyang
Oct 28, 2019·Physiological Reports·Jennifer A AguiarJeremy A Hirota
Dec 5, 2009·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Michael D JohnsonPeter G Shields
Dec 16, 2020·JGH Open : an Open Access Journal of Gastroenterology and Hepatology·Aoife CannonJacintha O'Sullivan

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