Development and evaluation of multiplex PCR assays for rapid detection of virulence-associated genes in Arcobacter species

Journal of Microbiological Methods
Jenni Whiteduck-LéveilléeIzhar U H Khan

Abstract

As the pathogenicity of Arcobacter species might be associated with various virulence factors, this study was aimed to develop and optimize three single-tube multiplex PCR (mPCR) assays that can efficiently detect multiple virulence-associated genes (VAGs) in Arcobacter spp. including the Arcobacter butzleri, Arcobacter cryaerophilus and Arcobacter skirrowii, respectively. The recognized target virulence factors used in the study were fibronectin binding protein (cj1349), filamentous hemagglutinin (hecA), hemolysin activation protein (hecB), hemolysin (tlyA), integral membrane protein virulence factor (mviN), invasin (ciaB), outer membrane protein (irgA) and phospholipase (pldA). Identical results were obtained between singleplex PCR and mPCR assays and no cross- and/or non-specific amplification products were obtained when tested against other closely related bacterial species. The sensitivities of these three mPCR assays were ranging from 1ngμL(-1) to 100ngμL(-1) DNA. The developed assays with combinations of duplex or triplex PCR primer pairs of VAGs were further evaluated and validated by applying them to isolates of the A. butzleri, A. cryaerophilus and A. skirrowii recovered from fecal samples of human and animal origins....Continue Reading

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May 16, 2015·International Journal of Systematic and Evolutionary Microbiology·Kerri Whiteduck-LéveilléeIzhar U H Khan

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Citations

Sep 1, 2017·Clinical Microbiology Reviews·Scott QuainooHeiman F L Wertheim
Apr 3, 2019·Annals of the New York Academy of Sciences·Winifred F FrickJon Flanders
Mar 30, 2017·Frontiers in Cellular and Infection Microbiology·Chang-Ro LeeSang Hee Lee
Dec 19, 2020·Comprehensive Reviews in Food Science and Food Safety·Daniele ChieffiVincenzina Fusco

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