Development and evaluation of novel tumor-targeting paclitaxel-loaded nano-carriers for ovarian cancer treatment: in vitro and in vivo

Journal of Experimental & Clinical Cancer Research : CR
Shu YaoKun Song

Abstract

Ovarian cancer is the most leading cause of death and the third most common gynecologic malignancy in women. Traditional chemotherapy has inevitable drawbacks of nonspecific tumor targeting, high toxicity, and poor therapeutic efficiency. In order to overcome such shortcomings, we prepared a novel nano-carrier drug-delivery system to enhance the anti-tumor efficiency. In vitro characterizations of nano-carriers were determined by TEM, DLS. Cell viability was measured by MTT method. RT-PCR was performed to measure the expression of FARα in three ovarian cancer cell lines. The drug-release study and the uptaken study were measured in vitro. The pharmacokinetic and the drug distribution study were verified by HPLC methods in vivo. The enhanced anti-tumor efficiency of FA-NP was evaluated by the tumor inhibitory rate in vivo. Paclitaxel (PTX)-loaded nanoparticles (NPs) (PTX-PEG-PLA-NP and PTX-PEG-PLA-FA-NP) were prepared successfully, and the drug-release study showed that the cumulative release rates of NP groups were much less than free PTX group. The pharmacokinetic study showed that the elimination phase of two kinds of NP groups were much longer than that of PTX group. The drug distribution in different tissues showed that the...Continue Reading

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Citations

Sep 10, 2019·Therapeutic Delivery·Vahid Taghipour-SabzevarMehrdad Moosazadeh Moghaddam
Oct 19, 2019·Artificial Cells, Nanomedicine, and Biotechnology·Swati GuptaSuresh P Vyas
Dec 24, 2019·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Chao YangHaitao Ran
Mar 15, 2019·Journal of Experimental & Clinical Cancer Research : CR·Barbara FrigerioMariangela Figini
Oct 19, 2020·International Journal of Pharmaceutics·Yuan LiHuile Gao
Dec 20, 2020·Life Sciences·Mahmood BaraniGeorge Z Kyzas
Aug 10, 2020·Chemico-biological Interactions·Yoke Ying TanDinesh Kumar Chellappan
Apr 16, 2021·Frontiers in Cell and Developmental Biology·Yuan MaGe Zhang
Aug 11, 2020·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Lina DongJingjing Li
Aug 25, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Raquel Santos FariaRicardo Bentes Azevedo
Dec 17, 2021·Recent Patents on Anti-cancer Drug Discovery·Debasmita MukhopadhyayVinod Paul

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Methods Mentioned

BETA
reverse transcription PCR
fluorescence microscopy
Fluorescence

Software Mentioned

Image J
SPSS

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