Development and evaluation of qualitative detection methods for unapproved genetically modified rice (LLRice)

Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan
Takahiro WatanabeTamio Maitani

Abstract

We developed a specific method to extract DNA from rice grain samples and modified the qualitative real-time PCR method provided by Bayer Co., Ltd. for reliable detection of the genetically modified (GM) rice variety, LLRice601, which has not undergone safety assessment for regulatory approval in Japan. Moreover, we conducted a data analysis to confirm the results obtained with real-time PCR. The yields of DNA extracted from powdered samples of rice grains were almost equal among 5 different varieties of rice, and there was no significant difference in the yield over three days. Reliable results were obtained using 50 ng of the extracted DNA as the template for real-time PCR. To examine the adequacy of the methods, we organized an interlaboratory study with the participation of 2 laboratories, in which 80 test samples were analyzed in a blinded manner. The statistical analysis revealed no significant difference in the Ct value for the endogenous gene of the DNA samples and for the targeted DNA sequence of 0.1% samples. The limit of detection of the method was approximately 0.1%. Analysis of the fluorescence intensity of the PCR-amplified product of the construct-specific DNA sequence suggested that it may be reasonable to judge...Continue Reading

References

Mar 21, 1998·Proceedings of the National Academy of Sciences of the United States of America·M D Bennett
Oct 2, 2001·Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan·T MatsuokaA Hino
May 7, 2003·Analytical and Bioanalytical Chemistry·Arne Holst-JensenKnut G Berdal
Jun 1, 2004·Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan·Chiseko WakuiTamio Maitani
Sep 2, 2004·Biological & Pharmaceutical Bulletin·Takahiro WatanabeMisao Kubo
Apr 20, 2006·Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan·Takahiro WatanabeTamio Maitani
Jan 25, 2007·Journal of Agricultural and Food Chemistry·Takahiro WatanabeTamio Maitani
May 11, 2010·Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan·Hiroshi AkiyamaReiko Teshima

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Citations

Nov 28, 2009·Analytical and Bioanalytical Chemistry·Philippe RaymondKerry R Emslie
Mar 28, 2009·Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan·Takahiro WatanabeRieko Matsuda
Jul 16, 2009·Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan·Taichi OguchiKazumi Kitta
Nov 10, 2009·Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan·Takahiro Watanabe, Rieko Matsuda
Mar 9, 2010·Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan·Kunihiko TakahashiMasakazu Horie
Jan 14, 2011·Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan·Hiroshi Akiyama, Kazumi Kitta
May 11, 2010·Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan·Hiroshi AkiyamaReiko Teshima
Jul 3, 2010·Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan·Taichi OguchiKazumi Kitta

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