Abstract
Owing to its diverse sex types, the cucumber plant has been studied widely as a model for sex determination. In addition to environmental factors and plant hormones, three major genes-F/f, M/m, and A/a-regulate the sex types in the cucumber plant. By combining the bulked segregant analysis (BSA) and the sequence-related amplified polymorphism (SRAP) technology, we identified eight markers linking to the M/m locus. Among them, the two closely linked SRAP markers flanking the M/m locus were the co-dominant marker ME1EM26 and the dominant marker ME1EM23. Further, the co-dominant marker ME8SA7 co-segregated with the M/m locus. With the chromosome walking method using the cucumber genomic bacterial artificial chromosome (BAC) library, we successfully developed a co-dominant SCAR marker S_ME1EM23 from the ME1EM23 sequence. Along with the other two co-dominant SCAR markers S_ME1EM26 and S_ME8SA7 (developed from ME1EM26 and ME8SA7, respectively) in a larger segregating population (900 individuals), the M/m locus was mapped between S_ME1EM26 (5.4 cM) and S_ME1EM23 (0.7 cM), and S_ME8SA7 co-segregated with it.
References
Nov 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·R W MichelmoreR V Kesseli
Jul 1, 1991·Analytical Biochemistry·B J BassamP M Gresshoff
Oct 1, 1987·Genomics·E S LanderL Newburg
Nov 11, 1995·Nucleic Acids Research·P VosM Kuiper
Mar 1, 1997·Plant Physiology·T TrebitshS D O'Neill
Sep 1, 1997·Nucleic Acids Research·S F AltschulD J Lipman
Jan 22, 1998·Plant & Cell Physiology·S KamachiS Sakai
Mar 17, 2001·The Plant Cell·M M KaterG C Angenent
Jun 28, 2001·Plant & Cell Physiology·S YamasakiH Takahashi
Jul 2, 2003·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·G LiC F Quiros
Jul 8, 2003·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·M FerriolF Nuez
Aug 5, 2003·Planta·Yu-Jin HaoShu-Nong Bai
Apr 16, 2004·The Plant Cell·Milos Tanurdzic, Jo Ann Banks
Oct 19, 2004·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·H Mibus, T Tatlioglu
Jan 15, 2005·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·F J NogueraR Lozano
Aug 12, 2005·Science in China. Series C, Life Sciences·Gang WangRun Cai
Sep 1, 2005·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Diro Terefe, Turan Tatlioglu
Feb 24, 2006·Vitamins and Hormones·Seiji YamasakiHideyuki Takahashi
Aug 5, 2006·Plant & Cell Physiology·Ronit Rimon Knopf, Tova Trebitsh
Jul 17, 2007·Journal of Experimental Botany·Sayoko SaitoHideyuki Takahashi
Sep 12, 2007·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Mukhlesur RahmanGenyi Li
Citations
Oct 23, 2009·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Weiwei ZhangRun Cai
May 21, 2011·BMC Genomics·Wei LiGenyi Li
May 3, 2013·PloS One·Zheng LiZhen-Hui Gong
May 29, 2009·Genetics·Zheng LiRun Cai
Jun 23, 2010·Yi chuan = Hereditas·Qian-Yi TaoRun Cai
May 12, 2012·Journal of Experimental Botany·Zheng LiRun Cai
Oct 5, 2015·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Fengjiao BuSanwen Huang
Sep 10, 2015·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Junyi TanZheng Li
May 19, 2017·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Shanshan HouZheng Li
Apr 17, 2018·Journal of Experimental Botany·Qianyi TaoZheng Li
Jul 15, 2009·The New Phytologist·Neil JonesIzolda Pašakinskienė
Feb 3, 2019·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Khin Thanda WinSanghyeob Lee
Mar 21, 2020·Horticulture Research·Zheng LiYiqun Weng
Aug 30, 2018·Frontiers in Plant Science·Jian PanRun Cai
Mar 16, 2021·Horticulture Research·Zheng LiYiqun Weng