Development and fine mapping of three co-dominant SCAR markers linked to the M/m gene in the cucumber plant (Cucumis sativus L.).

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik
Zheng LiRun Cai

Abstract

Owing to its diverse sex types, the cucumber plant has been studied widely as a model for sex determination. In addition to environmental factors and plant hormones, three major genes-F/f, M/m, and A/a-regulate the sex types in the cucumber plant. By combining the bulked segregant analysis (BSA) and the sequence-related amplified polymorphism (SRAP) technology, we identified eight markers linking to the M/m locus. Among them, the two closely linked SRAP markers flanking the M/m locus were the co-dominant marker ME1EM26 and the dominant marker ME1EM23. Further, the co-dominant marker ME8SA7 co-segregated with the M/m locus. With the chromosome walking method using the cucumber genomic bacterial artificial chromosome (BAC) library, we successfully developed a co-dominant SCAR marker S_ME1EM23 from the ME1EM23 sequence. Along with the other two co-dominant SCAR markers S_ME1EM26 and S_ME8SA7 (developed from ME1EM26 and ME8SA7, respectively) in a larger segregating population (900 individuals), the M/m locus was mapped between S_ME1EM26 (5.4 cM) and S_ME1EM23 (0.7 cM), and S_ME8SA7 co-segregated with it.

References

Jul 1, 1991·Analytical Biochemistry·B J BassamP M Gresshoff
Nov 11, 1995·Nucleic Acids Research·P VosM Kuiper
Sep 1, 1997·Nucleic Acids Research·S F AltschulD J Lipman
Jun 28, 2001·Plant & Cell Physiology·S YamasakiH Takahashi
Jul 2, 2003·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·G LiC F Quiros
Jul 8, 2003·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·M FerriolF Nuez
Apr 16, 2004·The Plant Cell·Milos Tanurdzic, Jo Ann Banks
Oct 19, 2004·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·H Mibus, T Tatlioglu
Jan 15, 2005·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·F J NogueraR Lozano
Sep 1, 2005·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Diro Terefe, Turan Tatlioglu
Feb 24, 2006·Vitamins and Hormones·Seiji YamasakiHideyuki Takahashi
Jul 17, 2007·Journal of Experimental Botany·Sayoko SaitoHideyuki Takahashi
Sep 12, 2007·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Mukhlesur RahmanGenyi Li

❮ Previous
Next ❯

Citations

Oct 23, 2009·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Weiwei ZhangRun Cai
Jun 23, 2010·Yi chuan = Hereditas·Qian-Yi TaoRun Cai
Oct 5, 2015·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Fengjiao BuSanwen Huang
Sep 10, 2015·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Junyi TanZheng Li
May 19, 2017·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Shanshan HouZheng Li
Jul 15, 2009·The New Phytologist·Neil JonesIzolda Pašakinskienė
Feb 3, 2019·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Khin Thanda WinSanghyeob Lee

❮ Previous
Next ❯

Related Concepts

Related Feeds

Artificial Chromosomes

Artificial chromosomes are genetically engineered chromosomes derived from the DNA of a species. Discover the latest research on artificial chromosomes here.