Development and initial characterization of xenomitochondrial mice

Journal of Bioenergetics and Biomembranes
I TrounceC A Pinkert

Abstract

Xenomitochondrial mice harboring trans-species mitochondria on a Mus musculus domesticus (MD) nuclear background were produced. We created xenomitochondrial ES cell cybrids by fusing Mus spretus (MS), Mus caroli (MC), Mus dunni (Mdu), or Mus pahari (MP) mitochondrial donor cytoplasts and rhodamine 6-G treated CC9.3.1 or PC4 ES cells. The selected donor backgrounds reflected increasing evolutionary divergence from MD mice and the resultant mitochondrial-nuclear mismatch targeted a graded respiratory chain defect. Homoplasmic (MS, MC, Mdu, and MP) and heteroplasmic (MC) cell lines were injected into MD ova, and liveborn chimeric mice were obtained (MS/MD 18 of 87, MC/MD 6 of 46, Mdu/MD 31 of 140, and MP/MD l of 9 founder chimeras, respectively). Seven MS/MD, 1 MC/MD, and 11 Mdu/MD chimeric founder females were mated with wild-type MD males, and 18 of 19 (95%) were fertile. Of fertile females, only one chimeric MS/MD (1% coat color chimerism) and four chimeric Mdu/MD females (80-90% coat color chimerism) produced homoplasmic offspring with low efficiency (7 of 135; 5%). Four male and three female offspring were homoplasmic for the introduced mitochondrial backgrounds. Three male and one female offspring proved viable. Generation o...Continue Reading

Citations

Sep 13, 2007·Stem Cell Reviews·R Tayfur TecirliogluAlan O Trounson
Feb 2, 2016·Seminars in Cell & Developmental Biology·Justin C St John
Aug 23, 2011·Biochimica Et Biophysica Acta·David A DunnCarl A Pinkert
Jun 19, 2008·Annals of the New York Academy of Sciences·Austin L Hughes
Nov 20, 2013·Biochimica Et Biophysica Acta·Rachel Blake, Ian A Trounce
Mar 1, 2012·Pharmacology, Biochemistry, and Behavior·Kodeeswaran ParameshwaranCarl A Pinkert
Oct 26, 2005·Molecular Reproduction and Development·Kumiko TakedaAkira Onishi
Apr 4, 2006·Human Reproduction Update·E C SpikingsJ C St John
Aug 3, 2011·Reproductive Medicine and Biology·Matthew V CannonCarl A Pinkert

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacterial Respiration

This feed focuses on cellular respiration in bacteria, known as bacterial respiration. Discover the latest research here.