Development and inter-laboratory evaluation of real-time PCR assays for the detection of pospiviroids

Journal of Virological Methods
Wendy MongerSteen Lykke Nielsen

Abstract

Assays based on real-time PCR (TaqMan) that can detect a number of viroids in the genus Pospiviroid have been developed and evaluated. The assays are designed for detecting viroids from tomato leaf material but detection from other solanaceous hosts of these viroids has been confirmed. These methods have been validated by nine laboratories and comprise a reliable set of assays for the detection of CEVd, TASVd, CLVd and a generic assay which will detect the six viroids of concern to European tomato growers: PSTVd, TCDVd, CEVd, CLVd, TASVd and CSVd.

References

Jan 24, 2004·Journal of Virological Methods·N BoonhamR A Mumford
Mar 26, 2004·Journal of Virological Methods·Rick MumfordNeil Boonham
Jul 30, 2005·Journal of Virological Methods·N BoonhamR A Mumford

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Citations

Mar 4, 2014·Archives of Virology·Thibaut OlivierStéphan Steyer
Dec 3, 2014·Journal of Virological Methods·N Van BogaertG Smagghe
Nov 13, 2012·Journal of Virological Methods·Dongmei JiangLianhui Xie
Sep 18, 2012·Journal of Virological Methods·M BotermansE T M Meekes
Dec 18, 2013·Applied and Environmental Microbiology·N MehleM Ravnikar
Mar 17, 2017·Journal of Virological Methods·Fatima OsmanGeorgios Vidalakis

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