Development and optimization of a tumor targeting system based on microbial synthesized PHA biopolymers and PhaP mediated functional modification

Applied Microbiology and Biotechnology
Fan FanXiao-Yun Lu

Abstract

Polyhydroxyalkanoate (PHA) is a class of microbial synthesized biodegradable and biocompatible aliphatic polymer which has been developed into nanoparticles (NPs) for sustained release of hydrophobic compounds. Taking advantage of the natural PHA binding protein PhaP which could be steadily adsorbed onto PHA NPs through hydrophobic interaction, a tumor targeting system was developed in this study by presenting an epidermal growth factor receptor (EGFR)-targeting peptide (ETP) on the surface of PHA NPs, via PhaP mediated adsorption. To reveal the effects of residual emulsifiers on PhaP mediated ETP modification and optimize the tumor targeting capacity of the system, a novel emulsifier-free PHA NPs (EF-NPs) was fabricated together with other two kinds of conventional emulsifier-required PHA NPs (PVA-NPs and P68-NPs, which were prepared with poly(vinyl alcohol) (PVA) and Pluronic F68 as emulsifiers, respectively). By analyzing the surface hydrophobicity, the amount of adsorbed fusion protein, and the cellular uptake of all kinds of PHA NPs, our results demonstrated that EF-NPs with stronger surface hydrophobicity were the most proper formulation for further PhaP mediated ETP functionalization. The residual PVA and Pluronic F68 af...Continue Reading

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Citations

Apr 19, 2020·Applied and Environmental Microbiology·Aranzazu MatoM Auxiliadora Prieto
May 8, 2021·Frontiers in Chemistry·Kumar Sachin, Santosh Kumar Karn
Jul 3, 2021·Nanomaterials·Francisco G BlancoM Auxiliadora Prieto
Dec 26, 2018·Langmuir : the ACS Journal of Surfaces and Colloids·Aranzazu MatoM Auxiliadora Prieto

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Methods Mentioned

BETA
light
circular dichroism
fluorescence microscopy
xenograft
saturation binding

Software Mentioned

PyMol
Jasco
YASARA Energy Minimization Server
Modeler

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