PMID: 9523771Apr 2, 1998Paper

Development and pharmacological characterization of a modified procedure for the measurement of carbonic anhydrase activity

Journal of Pharmacological and Toxicological Methods
C LandolfiC Milanese

Abstract

Carbonic anhydrases (CAs) are a family of zinc metalloenzymes of molecular mass 30-60 kDa; seven different isoenzymes belong to this family (Okuyama et al., 1992, Proc Natl Acad Sci USA 89:1315-1319). They may be broadly recognized according to the efficiency with which they catalyze the reversible interconversion of CO2 and HCO3-, and they differ in physicochemical properties, in sensitivity to various inhibitors and in their subcellular localization; cytoplasmic (CA I, CA II, CA III, and CA VII), cell-surface membrane (CA IV), and mitochondrial (CA V) and secretory (CA VI) isoenzymes have been described. Several methods are reported in the literature for the measure of CA enzymatic activity; they may be broadly divided into two categories: those based on the measure of pH variation (pH-stat and colorimetric assays) (Wu et al., 1993, J Ocular Pharm 9:97-108; Maren, 1991, Molec Pharmacol 41:419-426) and the ones in which CO2 production is measured through pCO2 sensors (Botrè and Botrè, 1990, Anal Biochem 185:254-264).

References

Feb 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·T OkuyamaW S Sly
Jul 10, 1992·Journal of Medicinal Chemistry·S AntonaroliG Segre
Mar 1, 1990·Analytical Biochemistry·C Botrè, F Botrè

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Citations

Jun 9, 1999·Proceedings of the National Academy of Sciences of the United States of America·M K SunD L Alkon
Nov 25, 2006·The Journal of Physiology·Bernardo V AlvarezJoseph R Casey
Sep 11, 2012·Journal of Enzyme Inhibition and Medicinal Chemistry·Nurgün BüyükkidanBülent Büyükkidan
Sep 22, 2012·Journal of Enzyme Inhibition and Medicinal Chemistry·Nurgün BüyükkidanHatice Gonca Yalçin
Aug 19, 2011·Journal of Enzyme Inhibition and Medicinal Chemistry·Muharrem KayaMetin Bülbül
Apr 4, 2002·Clinical Pharmacokinetics·Jens Martens-Lobenhoffer, Peter Banditt
Feb 8, 2007·American Journal of Physiology. Endocrinology and Metabolism·Abdullah SenerWilly J Malaisse
Sep 24, 2010·Journal of Enzyme Inhibition and Medicinal Chemistry·Cengiz YenikayaOrhan Büyükgüngör
Jun 18, 2010·Journal of Enzyme Inhibition and Medicinal Chemistry·Rahmi KasımoğullarıHülya Güleryüz
Apr 14, 2009·Bioorganic & Medicinal Chemistry·Rahmi KasimoğullariHülya Güleryüz
Dec 17, 2009·Bioorganic & Medicinal Chemistry·Cengiz YenikayaOrhan Büyükgüngör
Aug 21, 2010·European Journal of Medicinal Chemistry·Rahmi KasımoğullarıBaşak Gökçe

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