Development and quality of sheep embryos cultured in commercial G1.3/G2.3 sequential media

Animal Reproduction Science
R M Garcia-GarciaP Lonergan

Abstract

Present study assesses the developmental ability and quality of ovine IVP embryos derived from culture in sequential media G1.3/G2.3. A total of 1474 cumulus-oocyte complexes were matured in M199 supplemented with EGF and FCS for 24h in 5% CO2 in humidified air at 39 degrees C. Oocytes were co-incubated in SOF medium with 1 x 10(6) spermatozoa/ml at the same temperature and gas conditions (Day 0 p.i.). Presumptive zygotes at 20 h p.i. were denuded, washed and placed in culture in SOF (control; n=742) or G1.3 media supplemented with 3mg/ml of BSA (n=732) under mineral oil in a humidified and controlled atmosphere at 39 degrees C. Embryos in the treated group were changed to G2.3 medium on Day 3 of culture. A group of blastocysts in each group were frozen by conventional method (SOF, n=55; G1.3/G2.3, n=48). In vivo embryos (n=72) were recovered at Day 7 from the uterus of progestagen+eCG treated females and they were cultured in defined medium (n=38) or frozen (n=34) directly after recovery. Cleavage rate of IVP embryos recorded at 48 h p.i. was similar for control and treated embryos (49.8 versus 47.5%). There were no significant differences in blastocyst development from the two groups on Day 6 (26.0 versus 25.6%), 7 (42.1 vers...Continue Reading

References

Mar 1, 1996·Journal of Reproduction and Fertility·J G ThompsonH J Leese
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Aug 24, 1999·Biology of Reproduction·T E Steeves, D K Gardner
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Citations

Apr 1, 2014·Journal of Assisted Reproduction and Genetics·V A Absalón-MedinaR O Gilbert
Jul 13, 2012·Zygote : the Biology of Gametes and Early Embryos·Xian-rong XiongYong Zhang
Mar 22, 2014·Theriogenology·Joanna Maria Gonçalves de Souza-FabjanPascal Mermillod
Jul 29, 2010·Reproduction in Domestic Animals = Zuchthygiene·Y S WangY Zhang
Oct 4, 2014·Reproduction in Domestic Animals = Zuchthygiene·M-T Paramio, D Izquierdo
Dec 31, 2014·Journal of Veterinary Science·Dong-Hoon KimHong-Tea Kim

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