Development and Symbiosis Establishment in the Cnidarian Endosymbiosis Model Aiptasia sp

Scientific Reports
Madeline BucherAnnika Guse

Abstract

Symbiosis between photosynthetic algae and heterotrophic organisms is widespread. One prominent example of high ecological relevance is the endosymbiosis between dinoflagellate algae of the genus Symbiodinium and reef-building corals, which typically acquire symbionts anew each generation during larval stages. The tropical sea anemone Aiptasia sp. is a laboratory model system for this endosymbiosis and, similar to corals, produces non-symbiotic larvae that establish symbiosis by phagocytosing Symbiodinium from the environment into the endoderm. Here we generate the first overview of Aiptasia embryogenesis and larval development and establish in situ hybridization to analyze expression patterns of key early developmental regulators. Next, we quantify morphological changes in developing larvae and find a substantial enlargement of the gastric cavity over time. Symbiont acquisition starts soon after mouth formation and symbionts occupy a major portion of the host cell in which they reside. During the first 14 days of development, infection efficiency remains constant while in contrast, localization of phagocytosed symbionts changes, indicating that the occurrence of functional phagocytosing cells may be developmentally regulated. ...Continue Reading

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Citations

Sep 2, 2016·Scientific Reports·Iliona WolfowiczAnnika Guse
Mar 23, 2019·Annals of the New York Academy of Sciences·James A Shapiro
Nov 11, 2020·Proceedings of the National Academy of Sciences of the United States of America·Phillip A ClevesJohn R Pringle
Nov 24, 2020·Molecular Biology and Evolution·Sebastian G GornikAnnika Guse
May 1, 2021·Nature Microbiology·Marie R JacobovitzAnnika Guse
Aug 7, 2021·Genes·Chloé A van der Burg, Peter J Prentis

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Methods Mentioned

BETA
confocal microscopy
chip
PCR
Fluorescence

Software Mentioned

Nikon Elements Software
Leica Application Suite Advanced Fluorescence
Fiji

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