Development and use of an indirect enzyme-linked immunosorbent assay for detection of iridovirus exposure in gopher tortoises (Gopherus polyphemus) and eastern box turtles (Terrapene carolina carolina)

Veterinary Microbiology
April J JohnsonE R Jacobson

Abstract

Iridoviruses, pathogens typically associated with fish and amphibians, have recently been shown to cause acute respiratory disease in chelonians including box turtles, red-eared sliders, gopher tortoises, and Burmese star tortoises. Case reports of natural infections in several chelonian species in the United States have been reported, however the prevalence remains unknown in susceptible populations of free-ranging chelonians. To determine the prevalence of iridovirus exposure in free-ranging gopher tortoises (Gopherus polyphemus) in the southeast United States, an indirect enzyme-linked immunosorbent assay (ELISA) was developed and used to evaluate plasma samples from wild gopher tortoises (G. polyphemus) from: Alabama (n=9); Florida (n=658); Georgia (n=225); Louisiana (n=12); Mississippi (n=28); and unknown locations (68) collected between 2001 and 2006. Eight (1.2%) seropositive tortoises were identified from Florida and seven (3.1%) from Georgia for an overall prevalence of 1.5%. Additionally, a population of eastern box turtles was sampled from a private nature sanctuary in Pennsylvania that experienced an outbreak of iridovirus the previous year, which killed 16 turtles. Only 1 turtle out of 55 survivors tested positive ...Continue Reading

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Citations

Apr 10, 2014·Journal of Zoo and Wildlife Medicine : Official Publication of the American Association of Zoo Veterinarians·Samanta WaxmanCasilda Rodríguez
Sep 22, 2011·Veterinary Research·Ellen Ariel
Dec 17, 2011·Emerging Infectious Diseases·Wuzi DongWenxian Zeng
Dec 17, 2015·Journal of Zoo and Wildlife Medicine : Official Publication of the American Association of Zoo Veterinarians·Jennifer C HausmannEllen Bronson
Jun 5, 2014·Journal of Aquatic Animal Health·Roberto BrenesMatthew J Gray
Apr 21, 2017·Diseases of Aquatic Organisms·Mickey AghaBrian D Todd
Nov 22, 2017·Diseases of Aquatic Organisms·E ArielL Owens
Jul 28, 2017·Journal of Zoo and Wildlife Medicine : Official Publication of the American Association of Zoo Veterinarians·Ekaterina KolesnikRachel E Marschang
Dec 26, 2018·PeerJ·Wytamma WirthEllen Ariel
Apr 25, 2020·The Veterinary Clinics of North America. Exotic Animal Practice·Laura AdamoviczPaul M Gibbons
Apr 25, 2021·The Veterinary Clinics of North America. Exotic Animal Practice·Rachel E MarschangMichael Pees
Oct 9, 2021·Frontiers in Veterinary Science·Kate ParrishEllen Ariel

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