Development and use of microsatellite markers to study diversity, reproduction and population genetic structure of the cereal pathogen Ramularia collo-cygni

Fungal Genetics and Biology : FG & B
M J PiotrowskaP N Hoebe

Abstract

Ramularia collo-cygni (Rcc) is a major pathogen of barley that causes economically serious yield losses. Disease epidemics during the growing season are mainly propagated by asexual air-borne spores of Rcc, but it is thought that Rcc undergoes sexual reproduction during its life cycle and may also disperse by means of sexual ascospores. To obtain population genetic information from which to infer the extent of sexual reproduction and local genotype dispersal in Rcc, and by implication the pathogen's ability to adapt to fungicides and resistant cultivars, we developed ten polymorphic microsatellite markers, for which primers are presented. We used these markers to analyse the population genetic structure of this cereal pathogen in two geographically distant populations from the Czech Republic (n=30) and the United Kingdom (n=60) that had been sampled in a spatially explicit manner. Genetic diversity at the microsatellite loci was substantial, Ht=0.392 and Ht=0.411 in the Czech and UK populations respectively, and the populations were moderately differentiated at these loci (Θ=0.111, P<0.01). In both populations the multilocus genotypic diversity was very high (one clonal pair per population, resulting in >96% unique genotypes in...Continue Reading

References

Oct 1, 1975·Theoretical Population Biology·A H Brown
May 26, 1999·Proceedings of the National Academy of Sciences of the United States of America·D Tilman
Feb 5, 2000·Nature Biotechnology·M Schuelke
Apr 27, 2000·Trends in Ecology & Evolution·P Sunnucks
Nov 10, 2000·Chromosoma·C Schlötterer
Jul 31, 2002·Annual Review of Phytopathology·Bruce A McDonald, Celeste Linde
Feb 28, 2006·FEMS Microbiology Letters·Neil D HavisStephen R H Langrell
Apr 29, 2006·Ecology Letters·Kimberly A Selkoe, Robert J Toonen
Dec 12, 2007·FEMS Microbiology Letters·Dale R WaltersSimon J P Oxley
Aug 6, 2008·Annual Review of Phytopathology·Eva H Stukenbrock, Bruce A McDonald
Oct 24, 2008·Phytopathology·B A McDonaldJ J Burdon
Oct 25, 2008·Trends in Ecology & Evolution·Luke G BarrettCeleste C Linde
Sep 23, 2009·Pest Management Science·Derek W Hollomon, Keith J Brent
Jul 14, 2010·Molecular Ecology·Roman Biek, Leslie A Real
Oct 1, 1996·Trends in Ecology & Evolution·P Jarne, P J Lagoda
Sep 1, 2008·Molecular Ecology Resources·I BarnesB D Wingfield
Jun 26, 2012·Nucleic Acids Research·Andreas UntergasserSteven G Rozen

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