Development and utility of the FDA 'GutProbe' DNA microarray for identification, genotyping and metagenomic analysis of commercially available probiotics

Journal of Applied Microbiology
J N PatroC A Elkins

Abstract

Lactic acid bacteria are beneficial microbes added to many food products and dietary supplements for their purported health benefits. Proper identification of bacteria is important to assess safety as well as proper product labelling. A custom microarray (FDA GutProbe) was developed to verify accurate labelling in commercial dietary supplements. Strain-specific attribution was achieved with GutProbe array which contains genes from the most commonly found species in probiotic supplements and food ingredients. Applied utility of the array was assessed with direct from product DNA hybridization to determine (i) if identification of multiple strains in one sample can be conducted and (ii) if any lot-to-lot variations exist with eight probiotics found on the US market. GutProbe is a useful tool in identifying a mixture of microbials in probiotics and did reveal some product variations. In addition, the array is able to identify lot-to-lot differences in these products. These strain level attribution may be useful for routine monitoring of batch variation as part of a 'Good Manufacturing Practices' process. The FDA GutProbe is an efficient and reliable platform to identify the presence of microbial ingredients and determining microbe...Continue Reading

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Citations

Sep 14, 2015·Journal of Chromatography. a·Monica Gallo, Pasquale Ferranti
Nov 17, 2015·Pediatric Research·Zachery T LewisDavid A Mills
Aug 25, 2016·BMC Genomics·Anne AbotVéronique Anton Leberre
Jul 21, 2017·Journal of Agricultural and Food Chemistry·Lin TaoYu Zong Chen
Sep 20, 2015·Applied and Environmental Microbiology·Susan R LeonardChristopher A Elkins
Apr 6, 2021·Critical Reviews in Food Science and Nutrition·Vincenzina FuscoDaniele Chieffi

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