Development and validation of a bioanalytical method for quantification of 2,6-bis-(4-hydroxy-3-methoxybenzylidene)-cyclohexanone (BHMC) in rat plasma

Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry
Yu Zhao LeeChau Ling Tham

Abstract

A sensitive and accurate high performance liquid chromatography with ultraviolet/visible light detection (HPLC-UV/VIS) method for the quantification of 2,6-bis-(4-hydroxy-3-methoxybenzylidene)-cyclohexanone (BHMC) in rat plasma was developed and validated. BHMC and the internal standard, harmaline, were extracted from plasma samples by a simple liquid-liquid extraction using 95% ethyl acetate and 5% methanol. Plasma concentration of BHMC and internal standard were analyzed by reversed phase chromatography using a C₁₈ column (150 × 4.6 mm I.D., particle size 5 µm) and elution with a gradient mobile phase of water and methanol at a flow rate of 1.0 mL/min. Detection of BHMC and internal standard was done at a wavelength of 380 nm. The limit of quantification was 0.02 µg/mL. The calibration curves was linear (R² > 0.999) over the concentration range of 0.02-2.5 µg/mL. Intra- and inter-day precision were less than 2% coefficient of variation. The validated method was then applied to a pharmacokinetic study in rats by intravenous administration of BHMC at a single dose of 10 mg/kg. Pharmacokinetic parameters such as half-life, maximum plasma concentration, volume of distribution, clearance and elimination rate constant for BHMC were...Continue Reading

References

Mar 1, 2003·Journal of Pharmaceutical and Biomedical Analysis·M KartalS Kurucu
Apr 3, 2007·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Kuo-Yi YangTung-Hu Tsai
Nov 15, 2007·Molecular Pharmaceutics·Preetha AnandBharat B Aggarwal
Sep 9, 2008·Biochemical Pharmacology·Preetha AnandBharat B Aggarwal
Dec 27, 2008·Trends in Pharmacological Sciences·Bharat B Aggarwal, Bokyung Sung
Dec 5, 2009·European Journal of Pharmacology·Chau Ling ThamDaud A Israf

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Citations

Oct 11, 2014·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Sze Wei LeongNordin H Lajis
Dec 1, 2018·Cancer Science·Sariya MapoungPornngarm Limtrakul Dejkriengkraikul
Jun 18, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Sariya MapoungPornngarm Limtrakul Dejkriengkraikul

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BETA
plasma collection

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Chromera Manager

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