Dec 30, 2016

Development and validation of a clinical HPLC method for the quantification of hydroxychloroquine and its metabolites in whole blood

Future Science OA
Ying QuBenoit Blanchet

Abstract

Therapeutic drug monitoring for hydroxychloroquine (HCQ) has been suggested to assess nonadherence and optimize treatment efficacy in systemic lupus erythematosus patients. After protein precipitation, HCQ and its metabolites, desethylhydroxychloroquine and desethylchloroquine were separated on a phenyl column and monitored by fluorescence detection. The method was linear from 50 to 4000 ng/ml for HCQ. The intra-day and inter-day precision of HCQ, desethylhydroxychloroquine and desethylchloroquine ranged from 4.3 to 10.3%. LLOQ was 50 ng/ml for HCQ. The method is very practical and was applied to routinely monitor the steady state whole blood exposure of HCQ and its metabolites in systemic lupus erythematosus patients. It well correlated with our LC-MS/MS and another HPLC method.

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Mentioned in this Paper

Whole Blood
Phenyl
Hydroxychloroquine
Tandem Mass Spectrometry
Fluorescence Spectroscopy
High Pressure Liquid Chromatography Procedure
Whole Blood Specimen
Liquid Chromatography Mass Spectrometry
Cyclosporine/Hydroxychloroquine/Prednisone
Desethylchloroquine

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