Development and validation of a direct headspace GC-FID method for the determination of sevoflurane, desflurane and other volatile compounds of forensic interest in biological fluids: application on clinical and post-mortem samples

Journal of Separation Science
Leda KovatsiVictoria Samanidou

Abstract

A simple and reliable headspace GC-flame ionization detection (HS-GC-FID) method has been developed and validated for the simultaneous determination of seven volatile compounds of forensic interest: sevoflurane, desflurane, ethanol, methanol, 1-propanol, acetone and acetaldehyde. All seven compounds including acetonitrile (internal standard) eluted within 10 min and were well resolved with no endogenous interference. Good linearity was observed in the range of 1-12 mg/dL for both anesthetics and 2.5-40 mg/dL for the other five analytes. The method showed good precision, sensitivity and repeatability. Most of the analytes remained stable during the storage of samples at 4°C. Desflurane and acetone degraded (>10%), when the samples remained on the autosampler for more than 2 and 3 h, respectively. The method was finally applied on clinical and post-mortem blood and urine samples. The clinical samples were collected both from patients who underwent surgery, as well as from the occupationally exposed medical and nursing staff of the university hospital, working in the operating rooms. The hospital staff samples were found negative for all compounds, while the patients' samples were found positive for the anesthetic administered to ...Continue Reading

References

Jan 1, 1984·Canadian Anaesthetists' Society Journal·M YamashitaT Oyama
May 20, 1998·European Journal of Clinical Pharmacology·U Klotz, E Ammon
Mar 12, 2003·Medicine, Science, and the Law·R Iffland, A W Jones
May 29, 2004·The American Journal of Forensic Medicine and Pathology·Fumio Moriya, Yoshiaki Hashimoto
Sep 1, 2004·Journal of Clinical Forensic Medicine·A M TsatsakisM N Michalodimitrakis
Oct 6, 2004·Journal of Forensic Sciences·Olli LaaksoJaakko-Juhani Himberg
Mar 17, 2007·Clinical Toxicology : the Official Journal of the American Academy of Clinical Toxicology and European Association of Poisons Centres and Clinical Toxicologists·R PaasmaD Jacobsen
Apr 25, 2007·Forensic Science International : Synergy·Vassiliki A BoumbaTheodore Vougiouklakis
Nov 9, 2007·Journal of Analytical Toxicology·B LevineDavid Fowler
Dec 14, 2007·Drug and Alcohol Dependence·James P ZacnyMolly Sells
Apr 10, 2008·Journal of Analytical Toxicology·H Rachelle Wallage, James H Watterson
Jul 9, 2008·Clinical Toxicology : the Official Journal of the American Academy of Clinical Toxicology and European Association of Poisons Centres and Clinical Toxicologists·F Lee Cantrell
Oct 14, 2008·Legal Medicine·Grzegorz TeresińskiRoman Madro
Jan 30, 2010·Journal of Analytical Toxicology·Walter Martz
Feb 2, 2010·Advanced Emergency Nursing Journal·Deanna M McMahonKyle A Weant
Jul 1, 2010·Clinical Toxicology : the Official Journal of the American Academy of Clinical Toxicology and European Association of Poisons Centres and Clinical Toxicologists·Jeffrey Brent

Citations

Jul 10, 2013·Forensic Science International : Synergy·Jessica L Westland, Frank L Dorman
Nov 3, 2012·PloS One·Chrysa LithariChristos L Papadelis
Dec 16, 2017·International Archives of Occupational and Environmental Health·Abbas JafariMohammad Hajaghazadeh

Related Concepts

Ultane
Suprane
Chromatography, Gas-Liquid
Legal Medicine Discipline
Isoflurane
Methyl Ethers
Livor Mortis
Volatile Organic Compounds
Acetaldehyde
Acetone

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