Development and validation of a direct homologous quantitative sandwich ELISA for fathead minnow (Pimephales promelas) vitellogenin

Aquatic Toxicology
Janne K EidemAnders Goksøyr

Abstract

Vitellogenin (Vtg) is an established and sensitive endpoint for analysis of exposure to (anti-)oestrogens and their mimics in fish [Sumpter, J.P., 1995. Feminized responses in fish to environmental estrogens. Toxicol. Lett. 82, 737-742; Arukwe, A., Goksøyr, A., 2003. Eggshell and egg yolk proteins in fish: hepatic proteins for the next generation: oogenetic, population, and evolutionary implications of endocrine disruption. Comp. Hepatol. 2, 4. ]. In some instances, links have been drawn between high level induction of Vtg and adverse health effects in fish [Herman, R.L., Kincaide, H.L., 1988. Pathological effects of orally administered estradiol to rainbow trout. Aquaculture 72, 165-172; Schwaiger, J., Spieser, O.H., Bauer, C., Ferling, H., Mallow, U., Kalbfus, W., Negele, R.D., 2000. Chronic toxicity of nonylphenol and ethinyloestraiol: haematological and histopathological effects in juvenile common carp (Cyprinus carpio). Aquat. Toxicol. 51, 69-78]. The widespread use of Vtg as a biomarker has led to the development of a variety of assays to quantitatively measure Vtg concentrations in tissue samples from fish, and hence a need for a standardization of the performance criteria and validation of such assays [Goksøyr, A., Eide...Continue Reading

Citations

Jul 5, 2006·Environmental Health Perspectives·Thomas H HutchinsonCharles R Tyler
Feb 5, 2011·Diseases of Aquatic Organisms·Yu RaoHeping Dai
Mar 17, 2016·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Jun WangShaoguo Ru
Nov 26, 2011·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Henrik HolbechAnne Gourmelon
Sep 11, 2007·Environmental Pollution·Thomas R RainwaterScott T McMurry
Apr 14, 2012·Journal of Proteomics·Pedro M RodriguesFlemming Jessen
Mar 6, 2009·Rapid Communications in Mass Spectrometry : RCM·Alejandro M CohenJoseph H Banoub
Jun 24, 2018·Environmental Science and Pollution Research International·Mingyi ZhengShaoguo Ru

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