Development and validation of a gas chromatography/mass spectrometry method for the metabolic profiling of human colon tissue

Rapid Communications in Mass Spectrometry : RCM
Mainak MalEric C Y Chan

Abstract

In this study, a gas chromatography/mass spectrometry (GC/MS) method was developed and validated for the metabolic profiling of human colon tissue. Each colon tissue sample (20 mg) was ultra-sonicated with 1 mL of a mixture of chloroform/methanol/water in the ratio of 20:50:20 (v/v/v), followed by centrifugation, collection of supernatant, drying, removal of moisture using anhydrous toluene and finally derivatization using N-methyl-N-trifluoroacetamide (MSTFA) with 1% trimethylchlorosilane (TMCS). A volume of 1 microL of the derivatized mixture was injected into the GC/MS system. A total of 53 endogenous metabolites were separated and identified in the GC/MS chromatogram, all of which were selected to evaluate the sample stability and precision of the method. Of the identified endogenous metabolites 19 belonging to diverse chemical classes and covering a wide range of the GC retention times (Rt) were selected to investigate the quantitative linearity of the method. The developed GC/MS method demonstrated good reproducibility with intra- and inter-day precision within relative standard deviation (RSD) of +/-15%. The metabolic profiles of the intact tissue were determined to be stable (100 +/- 15%) for up to 90 days at -80 degree...Continue Reading

References

Aug 5, 2000·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·M E BollardJ K Nicholson
Feb 24, 1956·Science·O WARBURG
Oct 31, 2003·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·O M RooneyJ L Griffin
Dec 24, 2003·American Heart Journal·JoAnne Micale FoodyHarlan M Krumholz
Jul 2, 2004·Nature Reviews. Cancer·Julian L Griffin, John P Shockcor
Nov 24, 2004·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Jun YangQing Yang
Feb 4, 2005·Toxicological Sciences : an Official Journal of the Society of Toxicology·Donald G Robertson
Dec 15, 2005·Analytical Chemistry·Jiye AThomas Moritz
Jan 28, 2006·American Journal of Physiology. Gastrointestinal and Liver Physiology·Roberto MazzantiCecilia Giulivi
Mar 24, 2006·Molecular & Cellular Proteomics : MCP·Xuezhi BiChoy-Leong Hew
Sep 16, 2006·Biochimica Et Biophysica Acta·Daniel B LongleyPatrick G Johnston
Oct 18, 2006·Rapid Communications in Mass Spectrometry : RCM·Hilary J MajorIan D Wilson
Nov 30, 2006·British Journal of Neurosurgery·Vladimir PetrikMarios C Papadopoulos
Feb 3, 2007·Journal of Proteome Research·Eva Maria Lenz, Ian D Wilson
Apr 5, 2007·Nature Protocols·Jan LisecAlisdair R Fernie
Oct 11, 2007·Pharmacogenomics·David I EllisRoyston Goodacre
May 16, 2008·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Kishore K PasikantiE C Y Chan

❮ Previous
Next ❯

Citations

Dec 17, 2009·Analytical and Bioanalytical Chemistry·Hao WuXizhong Shen
Mar 16, 2013·Analytical and Bioanalytical Chemistry·Michael D WilliamsHerbert H Hill
Sep 14, 2013·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Lee Cheng PhuaEric Chun Yong Chan
May 19, 2010·Biomarkers in Medicine·G A Nagana GowdaTedros Bezabeh
Mar 11, 2011·World Journal of Gastroenterology : WJG·Jun-Duo HuJin-Lian Chen
Jul 27, 2012·Metabolomics : Official Journal of the Metabolomic Society·Y M TikunovR C H de Vos
Sep 29, 2011·Metabolomics : Official Journal of the Metabolomic Society·Jing BaiWei Ning Chen
Jan 17, 2016·Annual Review of Food Science and Technology·Suzanne D JohanningsmeierClaire M Klevorn
Jan 28, 2010·Analytica Chimica Acta·Rasmus MadsenJohan Trygg
Jul 15, 2015·Journal of Mass Spectrometry : JMS·Andrey SamokhinIgor Revelsky
May 7, 2015·Analytical and Bioanalytical Chemistry·Michael D WilliamsHerbert H Hill
Jan 16, 2014·Cancer Biology & Therapy·Lee Cheng PhuaEric Chun Yong Chan
Aug 10, 2010·Mutation Research·Prashant R NambiarVikas Misra
Dec 14, 2011·Journal of Proteome Research·Yu ChengWei Jia
Mar 16, 2019·Pharmaceutics·Tânia F G G CovaSandra C C Nunes
May 17, 2015·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Lian Yee Yip, Eric Chun Yong Chan
Apr 29, 2015·Rare Tumors·Alexander AugustynAdam C Yopp
Sep 1, 2013·Personalized Medicine·Xiaojiao ZhengWei Jia
Dec 3, 2017·Cancer Chemotherapy and Pharmacology·Lee Cheng PhuaEric Chun Yong Chan
Feb 15, 2020·Cancer & Metabolism·Daniel R GarzaBas E Dutilh
Aug 1, 2015·Metabolomics : Official Journal of the Metabolomic Society·David B LiesenfeldCornelia M Ulrich
Oct 8, 2013·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·David B LiesenfeldCornelia M Ulrich

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.