Development and validation of a high-performance liquid chromatography method for the determination of cocaine, its metabolites and ketamine

Journal of Applied Toxicology : JAT
Hany Z Rofael, Mohamed S Abdel-Rahman

Abstract

Cocaine abuse is an extensive problem in the USA. During the past decade, ketamine abuse also has emerged as a public health concern and is now considered a controlled substance. The prevalence of the simultaneous use of cocaine and ketamine has been shown to be high. Previous research indicates that ketamine affects the enzymes that metabolize cocaine. In order to investigate this pharmacokinetic interaction, it was necessary to identify and quantitate each compound. The aim of this study is to develop a method of detecting and resolving cocaine, its metabolites and ketamine. A new precise, accurate and sensitive reversed-phase high-performance liquid chromatography method has been developed and validated. This assay employed a phosphate-buffered aqueous mobile phase (pH 6.9) with an organic component consisting of acetonitrile and methanol and a C-18 column as stationary phase at 225 nm wavelength. Minimum detection limits were 5 ng ml(-1) for cocaine and 10 ng ml(-1) for benzoylecgonine, norcocaine and ketamine. Linearity was demonstrated over a broad range of concentration in plasma, with good sensitivity for ketamine, cocaine and cocaine metabolites.

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Citations

Sep 27, 2003·Phytochemical Analysis : PCA
Oct 7, 2009·Analytical and Bioanalytical Chemistry·María Jesús TaberneroMarcello Chiarotti
Apr 28, 2004·Forensic Science International : Synergy·Jack Y K Cheng, Vincent K K Mok
May 16, 2003·Toxicology·Hany Z RofaelMohamed S Abdel-Rahman
Mar 7, 2002·Toxicology Letters·Hany Z Rofael, Mohamed S Abdel-Rahman
Jan 11, 2003·Journal of Toxicology and Environmental Health. Part a·Hany Z Rofael, Mohamed S Abdel-Rahman
Jan 1, 2005·Toxicology Mechanisms and Methods·Thomas VisalliMohamed S Abdel-Rahman
Mar 27, 2007·European Journal of Pharmacology·Joshua P ParlamanGregg D Stanwood
Sep 17, 2009·Journal of Veterinary Pharmacology and Therapeutics·G SellersM D Givens
Sep 16, 2010·Journal of Veterinary Pharmacology and Therapeutics·G SellersM D Givens
Nov 10, 2010·Biomedical Chromatography : BMC·Stacy D Brown, Tyler C Melton
Apr 24, 2012·Drug Testing and Analysis·Kristina Yasmin RustThomas Kraemer
Nov 25, 2011·Drug Testing and Analysis·Jessica A AlbrightDouglas J Beussman
Oct 14, 2003·International Journal of Toxicology·H Z RofaelM S Abdel-Rahman
Aug 18, 2018·Oxidative Medicine and Cellular Longevity·Ypatios SpanidisDemetrios Kouretas
Aug 30, 2003·Analytical Chemistry·T A BrettellR Saferstein

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