Development and validation of a high performance liquid chromatography method to determine linezolid concentrations in pig pulmonary tissue

Clinical Chemistry and Laboratory Medicine : CCLM
Laura GuerreroDolors Soy

Abstract

Linezolid is the first synthetic compound of a new group of antimicrobials, the oxazolidinones, which inhibit protein synthesis. It shows a broad spectrum of activity against Gram positive organisms. With respect to its pharmacokinetics, linezolid shows a relatively high volume of distribution and good penetration into inflammatory fluids, bone, fat and muscle. A reversed-phase isocratic high-performance liquid chromatographic method for linezolid analysis in piglet pulmonary tissue is described. Tissue samples and controls were prepared in 1 x TBE (1 M Tris, 0.9 M boric acid, 0.01 M EDTA). The mobile phase consisted of 20% ultrafiltered water and 80% of (A) 15 mM potassium monohydrogen phosphate buffer (pH = 5) with (B) acetonitrile (80%/20%; v/v). Samples were homogenized and precipitated with HClO(4) 3% (1/1, v/v). The injection volume was 100 microL. Ofloxacin was used as an internal standard. The assay was linear over a linezolid concentration range: 1.6-100 microg/mL. The method provided good validation data (n = 15): inaccuracy (3.6%), intra and inter-day variability (4.2% and 5.2%, respectively), recovery (91.8%), limit of detection (0.8 microg/mL) and quantitation (1.6 microg/mL) and acceptable stability within 24 h in...Continue Reading

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Citations

Mar 29, 2012·Bioanalysis·Kerri M Smith, Yan Xu
Oct 11, 2016·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Samah A MohammedHytham M Ahmed
Apr 25, 2019·Critical Reviews in Analytical Chemistry·Guilherme Felipe Dos Santos FernandesJean Leandro Dos Santos
May 25, 2012·Critical Care Medicine·Laia Fernández-BaratAntoni Torres
Nov 9, 2012·Anesthesiology·Jean-Jacques RoubyUNKNOWN Nebulized Antibiotics Study Group
Apr 9, 2019·Critical Reviews in Analytical Chemistry·K S KokilambigaiJ Kavitha

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