Development and validation of a high-throughput method for the quantitative analysis of D-amphetamine in rat blood using liquid chromatography/MS3 on a hybrid triple quadrupole-linear ion trap mass spectrometer and its application to a pharmacokinetic study

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Nicola CesariSimone Braggio

Abstract

Amphetamines are a group of sympathomimetic drugs that exhibit strong central nervous system stimulant effects. D-Amphetamine ((+)-alpha-methylphenetylamine) is the parent drug in this class to which all others are structurally related. In drug discovery, d-amphetamine is extensively used either for the exploration of novel mechanisms involving the catecholaminergic system, or for the validation of new behavioural animal models. Due to this extensive use of D-amphetamine in drug research and its interest in toxicologic-forensic investigation, a specific and high-throughput method, with minimal sample preparation, is necessary for routine analysis of D-amphetamine in biological samples. We propose here a sensitive, specific and high-throughput bioanalytical method for the quantitative determination of D-amphetamine in rat blood using MS(3) scan mode on a hybrid triple quadrupole-linear ion trap mass spectrometer (LC-MS/MS/MS). Blood samples, following dilution with water, were prepared by fully automated protein precipitation with acetonitrile containing an internal standard. The chromatographic separation was achieved on a Waters XTerra C18 column (2.1mm x 30mm, 3.5microm) using gradient elution at a flow rate of 1.0mL/min over...Continue Reading

References

Aug 13, 1998·Journal of Chromatography. B, Biomedical Sciences and Applications·T Kraemer, H H Maurer
Aug 27, 2004·Journal of Mass Spectrometry : JMS·Gérard HopfgartnerLuc Alexis Leuthold
Aug 19, 2006·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Mitsuru KumihashiIwao Ijiri

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Citations

Jun 26, 2013·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Min SongSangkyu Lee
Sep 3, 2011·Journal of Pharmaceutical and Biomedical Analysis·Xiaowei ShiDeying Cao
Jul 24, 2013·Journal of Pharmaceutical and Biomedical Analysis·Assunta NapolitanoSonia Piacente
Mar 26, 2013·Methods : a Companion to Methods in Enzymology·Xiaoqian LiuJennifer E Van Eyk
Feb 12, 2014·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·Lukas VaclavikJeanne I Rader
Nov 15, 2017·Biomedical Chromatography : BMC·András Szeitz, Stelvio M Bandiera
Oct 11, 2011·Biochimica Et Biophysica Acta·Maureen A Kane

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