Development and validation of a highly efficient protocol of porcine somatic cloning using preovulatory embryo transfer in peripubertal gilts

Cloning and Stem Cells
Björn PetersenHeiner Niemann

Abstract

The efficiency of porcine somatic nuclear transfer (born piglets/transferred embryos) is low. Here, we report a highly efficient protocol using peripubertal gilts as recipients synchronized to ovulate approximately 24 h after transfer of cloned embryos. Retrospectively, we compared the efficiency of two different synchronization protocols: In group 1, recipient animals were synchronized to ovulate approximately 6 h prior to surgical embryo transfer while in group 2 the animals were treated to ovulate 24 h after embryo transfer. In total, 1562 cloned embryos were transferred to 12 recipients in group 1; two of them became pregnant (16.7%). One pregnancy was lost on day 32, the second pregnancy went to term, and led to the birth of one healthy piglet after Cesarean section. In group 2, 1531 cloned embryos were transferred to 12 recipients. Nine recipients (75.0%) became pregnant as determined by ultrasound scanning on day 25. All pregnancies went to term and delivered a total of 47 live-born piglets. The cloning efficiency of both groups differed significantly (group 1: 0.1%, group 2: 3.1%, p < 0.05). This modified protocol was then applied in subsequent experiments using different types of transgenic and nontransgenic donor cell...Continue Reading

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Citations

Jun 4, 2011·Reproduction, Fertility, and Development·M SchmidtJ S Agerholm
Sep 7, 2012·Reproduction, Fertility, and Development·Geon A KimByeong Chun Lee
Dec 4, 2010·Stem Cells and Development·Monika Nowak-ImialekHeiner Niemann
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Jul 7, 2011·Proceedings of the National Academy of Sciences of the United States of America·Janet HauschildHeiner Niemann
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Nov 28, 2013·Theriogenology·Christopher G Grupen
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Jan 1, 2010·Xenotransplantation·Björn PetersenHeiner Niemann
Sep 5, 2012·Animal Science Journal = Nihon Chikusan Gakkaihō·Kui HuZhonghua Liu
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Jan 22, 2015·Transgenic Research·Bjoern Petersen, Heiner Niemann
Feb 3, 2015·Animal Reproduction Science·Junsong ShiZicong Li
Jan 19, 2015·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Bjoern Petersen, Heiner Niemann
Sep 24, 2015·Reproduction, Fertility, and Development·Rong LiHenrik Callesen
Oct 22, 2016·Asian-Australasian Journal of Animal Sciences·Jae-Gyu YooSeong-Keun Cho
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May 24, 2019·Xenotransplantation·Hendrik Johannes SakeBjörn Petersen
Jun 28, 2011·Journal of Animal Science·H NiemannG Flachowsky
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Jun 5, 2019·Nature Cell Biology·Xuefei GaoPentao Liu
Aug 18, 2017·Reproduction in Domestic Animals = Zuchthygiene·Bjoern Petersen
May 21, 2020·Cellular Reprogramming·Monika Nowak-ImialekHeiner Niemann
May 18, 2021·Molecular Therapy. Methods & Clinical Development·Peng GuWeiwang Gu
Jun 29, 2021·Zygote : the Biology of Gametes and Early Embryos·Jongki ChoChung-Gyu Park

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