Abstract
Lenalidomide, an immunomodulatory agent, and flavopiridol, a broad cyclin-dependent kinase inhibitor, are active therapies for clinical use in genomic high-risk chronic lymphocytic leukemia. A high-performance liquid chromatographic assay with tandem mass spectrometric detection has been developed to simultaneously quantify lenalidomide and flavopiridol in human and mouse plasma to facilitate their combined clinical development. Samples were prepared by liquid-liquid extraction with acetonitrile (ACN)-containing internal standard, genistein, followed by evaporation of solvent and reconstitution in 95/5 H2O/ACN. Lenalidomide and internal standard were separated by reversed-phase liquid chromatography on a C-18 column using a gradient of H2O and ACN, each with 0.1% formic acid. Atmospheric pressure chemical ionization in positive ion mode with single reaction monitoring on a triple quadrupole mass spectrometer was applied to detect transitions of lenalidomide (260.06 > 149.10) and flavopiridol (402.09 > 341.02). Lower limits of quantification of lenalidomide and flavopiridol were 1 and 0.3 nM, respectively. Recoveries of lenalidomide and flavopiridol from human plasma ranged from 99% to 116% throughout their linear ranges. Within...Continue Reading
References
Sep 23, 1998·Cancer Chemotherapy and Pharmacology·S F StinsonT W Daw
Jun 22, 2001·Blood·F E DaviesK C Anderson
Dec 26, 2001·Leukemia·D GuptaK C Anderson
Sep 14, 2002·Biomedical Chromatography : BMC·Suoping ZhaiWilliam D Figg
Oct 18, 2002·Blood·Paul G RichardsonKenneth C Anderson
Apr 12, 2003·Leukemia & Lymphoma·Chris PepperPaul Bentley
Nov 4, 2004·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Tanyifor M TohnyaWilliam D Figg
Sep 28, 2006·Blood·John C ByrdMichael R Grever
Oct 6, 2006·The New England Journal of Medicine·Alan ListUNKNOWN Myelodysplastic Syndrome-003 Study Investigators
Nov 8, 2006·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Asher Chanan-KhanMyron S Czuczman
Dec 16, 2006·Current Cancer Drug Targets·A FerrajoliZ Estrov
Feb 20, 2007·Molecular Cancer Therapeutics·Roberto R RosatoSteven Grant
Oct 24, 2007·Journal of Clinical Pharmacology·Nianhang ChenOscar L Laskin
Dec 28, 2007·Investigational New Drugs·Premal H PatelRobert J Motzer
Apr 23, 2008·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Leslie A AndritsosJohn C Byrd
May 21, 2008·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Mitch A PhelpsJames T Dalton
Citations
Nov 20, 2008·Current Treatment Options in Oncology·Peter H Wiernik
Sep 6, 2008·Blood·Rosa LapalombellaJohn C Byrd
Oct 30, 2012·Chemistry Central Journal·Ibrahim A DarwishTilal E El-Samani
Mar 19, 2013·Chemistry Central Journal·Nasr Y KhalilAbdel-Rahman A Al-Majed
Jan 16, 2013·Chemistry Central Journal·Muzaffar IqbalIbrahim A Darwish
Jun 21, 2013·Journal of Hematology & Oncology·Jian HouJianmin Wang
Aug 10, 2011·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Craig C HofmeisterMitch A Phelps
Nov 13, 2010·PloS One·Wenjun NiMitch A Phelps
Jan 30, 2013·Expert Opinion on Pharmacotherapy·Peter H Wiernik
Jan 17, 2015·Journal of Pharmaceutical and Biomedical Analysis·Sridhar VeeraraghavanManivannan Rangasamy
Dec 4, 2013·Journal of Immunoassay & Immunochemistry·Nourah Z AlzomanReem M Abuhejail
Jul 15, 2016·Expert Opinion on Investigational Drugs·Sylvain GarciazRéda Bouabdallah
Jan 28, 2014·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Sridhar VeeraraghavanManivannan Rangasamy
Jan 24, 2019·British Journal of Clinical Pharmacology·Jim H HughesDavid J R Foster
Sep 15, 2012·Rapid Communications in Mass Spectrometry : RCM·Stefan LouwKelly Chibale
Jun 4, 2014·The American Journal of Tropical Medicine and Hygiene·Papichaya PhompraditKesara Na-Bangchang
Sep 8, 2019·Cancer Chemotherapy and Pharmacology·Jim H HughesDavid J R Foster
Feb 3, 2015·American Journal of Hematology·Kami MaddocksKristie A Blum
Jul 14, 2016·AIDS Research and Human Retroviruses·Valérie PourcherDominique Costagliola
Aug 10, 2021·The Analyst·Sierra Jackson, Abraham K Badu-Tawiah