Development and validation of a hydrophilic interaction chromatography-tandem mass spectrometry for quantification of nicotine and its metabolites in human maternal and cord sera

Biomedical Chromatography : BMC
Yusuke IwasakiHiroyuki Nakazawa

Abstract

A selective and sensitive HILIC-MS/MS method for the simultaneous quantification of nicotine and its metabolites in human maternal and cord sera was developed and validated. After solid-phase extraction, LC separation was achieved on a hydrophilic interaction chromatography. The validated method is capable of selective identification as well as accurate and sensitive quantification. Analyte recovery ranged from 86.2 to 107.7% and intra- and inter-day assay precision were less than 15% relative standard deviation. This sensitive HILIC-MS/MS method can be used to determine nicotine and its metabolic profile in smokers. This validated method is useful for the determination of nicotine and its metabolites in human serum in future studies of the effects of nicotine exposure on neonatal outcome.

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Citations

May 25, 2011·The Journal of Reproduction and Development·Yoshikazu AraiKunio Shiota
Jan 27, 2012·Rapid Communications in Mass Spectrometry : RCM·Dmitri V Zagorevski, Jennifer A Loughmiller-Newman
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Mar 4, 2014·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Hiroyuki Nakazawa
Jul 11, 2021·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Nadja MallockAndreas Luch

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