Development and validation of a hydrophilic interaction liquid chromatography-tandem mass spectrometry method for the quantification of the antidiabetic drug metformin and six others pharmaceuticals in wastewater

Journal of Chromatography. a
Reinhard OertelJulia Rossmann

Abstract

A rapid and sensitive hydrophilic interaction liquid chromatography (HILIC)-tandem mass spectrometry method was developed and fully validated for the simultaneous determination of the antidiabetic drug metformin and six further pharmaceuticals (atenolol, gabapentin, levofloxacin, ciprofloxacin, propranolol and trimethoprim) in influent and effluent wastewater. Five deuterated related compounds were used as internal standards in order to control the extraction, injection and ionization variability. Two solid phase extraction methods and Oasis HLB 1cc cartridge, using only 1mL sample with acid or basic pH-value were optimized and compared in order to match the specificity of both influent and effluent wastewater matrixes. The most important challenge was to efficiently extract the polar compound metformin from the aqueous matrix. This was possible by adjusting the sample pH to 10. On the other hand, the adjustment to acid pH prevents metformin binding on the SPE cartridges. Metformin was so directly recovered after passing through the cartridge and injected in the HPLC-MS/MS system without any other preparation. These two methods present advantages and drawbacks, but are both applicable to wastewater samples. The method with extr...Continue Reading

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