Development and validation of a LC-MS/MS method to determine sulforaphane in honey.

Food Chemistry
A M AresJosé L Bernal

Abstract

A new method was developed to determine sulforaphane (SFN) in honey using liquid chromatography tandem mass spectrometry (LC-MS/MS) with electrospray ionization (ESI). An efficient extraction procedure was proposed (average analyte recoveries were between 92% and 99%); this involved a solid phase extraction (SPE) with a polymeric sorbent. Chromatography was performed on a Synergi™ Hydro analytical column with a mobile phase of 0.02 M ammonium formate in water and acetonitrile, at a flow rate of 0.5 mL/min. The method was fully validated in terms of selectivity, limits of detection (LOD) and quantification (LOQ), linearity, carry-over effect, reinjection reproducibility, precision and accuracy. The LOD and LOQ values were below 0.8 μg/kg and 2.6 μg/kg, respectively. The proposed method was applied to analyze SFN in honey from different botanical origins (rosemary, multifloral, orange blossom and heather), and SFN was detected at trace levels in some of the honey samples examined.

References

Apr 20, 2001·Journal of Agricultural and Food Chemistry·N V MatusheskiE H Jeffery
Jun 13, 2006·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Shruti AgrawalThomas J Cook
Aug 4, 2009·Biomedical Chromatography : BMC·O N Campas-BaypoliJ López-Cervantes
May 13, 2010·Journal of Agricultural and Food Chemistry·Joon-Kwan MoonTakayuki Shibamoto
Nov 26, 2010·Journal of Agricultural and Food Chemistry·Pilar TruchadoFrancisco A Tomás-Barberán
Jun 16, 2011·International Journal of Molecular Sciences·Dandan Han, Kyung Ho Row
May 15, 2012·Trends in Molecular Medicine·Albena T Dinkova-Kostova, Rumen V Kostov
Jul 21, 2014·Food Chemistry·Ioannis K KarabagiasMichael G Kontominas

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Citations

Feb 2, 2016·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Ana M AresJosé Bernal
Dec 22, 2016·Critical Reviews in Food Science and Nutrition·Maria RussoSeyed Mohammad Nabavi

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