Development and validation of a liquid chromatography-mass spectrometry method for the quantitation of naltrexone and 6beta-naltrexol in guinea pig plasma

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Satyanarayana ValivetiAudra L Stinchcomb

Abstract

A quantitative liquid chromatographic-electrospray ionization mass spectrometry method for the determination of naltrexone and 6beta-naltrexol in guinea pig plasma has been developed and validated using naloxone as an internal standard. A single step precipitation-extraction technique was carried out to extract the plasma samples using acetonitrile:ethyl acetate (1:1, v/v). The chromatographic separation was performed on a C(18) column using a mobile phase consisting of 35:65 (v/v) acetonitrile:2 mM ammonium acetate with 0.01 mM ammonium citrate at a flow rate of 0.25 mL/min. The analyte was detected after positive electrospray ionization using selected ion monitoring (SIM) mode. The mean recoveries for naltrexone, naltrexol, and naloxone were 91.7, 89.3, and 99.0%, respectively. The lower limit of quantification (LLOQ) for naltrexone and 6beta-naltrexol was 1.25 ng/mL, and the limit of detection (LOD) was 0.75 ng/mL. The method was applied to a pharmacokinetic study in order to assess the drug disposition of naltrexone in guinea pigs.

References

Oct 16, 2002·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Barbara J MasonMichael G Boyeson

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Citations

Feb 9, 2011·Pharmaceutical Research·Stan L BanksAudra L Stinchcomb
Feb 6, 2008·Proceedings of the National Academy of Sciences of the United States of America·Daniel P WermelingAudra L Stinchcomb
Jul 22, 2005·Journal of Mass Spectrometry : JMS
Aug 6, 2014·International Journal of Biological Macromolecules·Mohammad-Bagher GholivandVahid Nasirian

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