Development and validation of a liquid chromatography-atmospheric pressure chemical ionization-tandem mass spectrometry method for simultaneous analysis of 10 amphetamine-, methamphetamine- and 3,4-methylenedioxymethamphetamine-related (MDMA) analytes in human meconium.

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Tamsin KellyMarilyn A Huestis

Abstract

A liquid chromatography-atmospheric pressure chemical ionization-tandem mass spectrometry (LC-APCI-MS/MS) method for quantification of 10 amphetamine-related analytes in 1g meconium is presented. Specimen preparation included homogenization and solid-phase extraction. Two multiple reaction monitoring transitions were monitored per analyte. Ten and 1 microL injection volumes permitted quantification up to 10,000 ng/g, with sufficient sensitivity to quantify minor metabolites. Lower limits of quantification ranged from 1.25 to 40 ng/g. Precision was less than 14.2%, with accuracy between 79 and 115%. Meconium from a methamphetamine-exposed neonate was analyzed. Metabolites p-hydroxymethamphetamine, norephedrine and 4-hydroxy-3-methoxymethamphetamine were identified in meconium for the first time.

References

Sep 5, 2003·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·S PichiniO García-Algar
Jul 2, 2004·Therapeutic Drug Monitoring·Rafael de la TorreJordi Camí
May 4, 2005·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·S PichiniO García-Algar
Jan 18, 2006·Clinica Chimica Acta; International Journal of Clinical Chemistry·Joey GareriGideon Koren

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Citations

Jan 7, 2009·Therapeutic Drug Monitoring·Teresa R GrayMarilyn A Huestis
Nov 10, 2010·Biomedical Chromatography : BMC·Stacy D Brown, Tyler C Melton
Feb 8, 2013·Drug Testing and Analysis·Terhi LauniainenIlkka Ojanperä
Dec 23, 2014·Bioanalysis·Ana Concheiro-Guisan, Marta Concheiro
Mar 5, 2013·Environment International·Sylwia NarkowiczJacek Namieśnik
Nov 25, 2016·Journal of Analytical Toxicology·Fang WuGwendolyn A McMillin
Feb 9, 2018·Therapeutic Drug Monitoring·Simuli L WabuyeleGwendolyn A McMillin
Mar 10, 2010·Rapid Communications in Mass Spectrometry : RCM·Noel W DaviesJohn J Ross
May 9, 2009·Analytical Chemistry·T A BrettellJ R Almirall

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