Development and validation of a liquid chromatography/tandem mass spectrometry method for the simultaneous quantification of serotonin and thromboxane B2 from activated platelets

International Journal of Laboratory Hematology
Valentine MinetLionel Pochet

Abstract

Availability of a rapid and reliable platelet activation assay avoiding limitations of current techniques would be valuable to diagnose heparin-induced thrombocytopenia and platelet secretion disorders. The first aim was to develop and validate an ultra-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method to quantify in a single run TxB2 synthesized and serotonin released from platelets. The second aim was to use our method in association with light transmission aggregometry (LTA) to select good platelet responders for the diagnosis of HIT. Electrospray ionization and chromatographic separation were optimized for the simultaneous dosage of serotonin and TxB2. The method was validated according to the European Medicines Agency (EMA) guideline for bioanalytical method validation. LTA was performed with monoclonal anti-CD9 (clone ALB6) as platelet activator to select good responders. Detection was performed using a tandem mass spectrometer with alternated positive and negative electrospray ionization. The total run time was 6 minutes. The method was validated for calibration curves, precision, accuracy, lower limit of quantification, carry-over, selectivity, and matrix effect. Platelet response to ALB6 w...Continue Reading

References

Feb 15, 2002·Reviews in Clinical and Experimental Hematology·A T Nurden, P Nurden
Feb 19, 2002·British Journal of Haematology·Elisabeth Maurer-SpurejDana V Devine
Apr 26, 2002·Anästhesiologie, Intensivmedizin, Notfallmedizin, Schmerztherapie : AINS·S KochJ Harenberg
Jan 25, 2005·Cellular and Molecular Life Sciences : CMLS·E Maurer-Spurej
Feb 9, 2010·Analytical and Bioanalytical Chemistry·Wilhelmina H A de JongIdo P Kema
Jul 28, 2011·British Journal of Haematology·Paul HarrisonUNKNOWN British Committee for Standards in Haematology
Jan 12, 2012·Thrombosis and Haemostasis·Marie-Christine Morel-KoppUNKNOWN ASTH Clinical Trials Group
Oct 2, 2012·Platelets·Gianmarco PoddaMarco Cattaneo
May 3, 2014·Journal of Pharmaceutical and Biomedical Analysis·Isabella SquellerioViviana Cavalca
Mar 4, 2015·Vascular Health and Risk Management·Rita PanicciaRosanna Abbate
Apr 17, 2015·Thrombosis and Haemostasis·Andrew D MumfordDiego Mezzano
Nov 7, 2015·International Journal of Laboratory Hematology·N K Sono-KoreeK J Smock
Nov 11, 2015·Clinical Chemistry·Paul J Jannetto, Robert L Fitzgerald
Mar 12, 2016·Expert Opinion on Drug Safety·Tamam Bakchoul
Nov 20, 2016·Journal of Thrombosis and Haemostasis : JTH·M-C Morel-KoppUNKNOWN subcommittee on platelet immunology
Apr 12, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Valentine MinetFrançois Mullier

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