Development and validation of a method for the quantitation of Delta9tetrahydrocannabinol in oral fluid by liquid chromatography electrospray-mass-spectrometry

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Marta ConcheiroManuel López-Rivadulla

Abstract

Analysis of Delta(9)tetrahydrocannabinol (Delta(9)THC) and its metabolites in biological samples is of great relevance for forensic purposes. In the case of oral fluid (OF), the analysis should determine Delta(9)THC, whereas in urine, it detects the inactive metabolite tetrahydrocannabinol carboxylic acid (THC-COOH). Most laboratories analyze Delta(9)THC in such samples using GC-MS methods, but these procedures are time-consuming and involve unavoidable previous extraction and derivatization. No data is yet available on the application of liquid chromatography-mass-spectrometry to detect Delta(9)THC in oral fluid. We report a validation method in which the Delta(9)THC is isolated from oral fluid by a simple liquid-liquid extraction with hexane and subsequently analyzed by liquid chromatography-mass-spectrometry. The method here reported for the determination of Delta(9)THC in oral fluid only requires 200 microl of sample and achieves limits of detection of 2 ng/ml, and has been used to analyze oral fluid samples collected from current drug users.

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Citations

Apr 4, 2007·Analytical and Bioanalytical Chemistry·Nele SamynGert De Boeck
Jun 16, 2011·Analytical and Bioanalytical Chemistry·Sabina Strano-RossiMarcello Chiarotti
Feb 26, 2013·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·D Di CorciaM Vincenti
Sep 12, 2009·Clinical Chemistry·Wendy M Bosker, Marilyn A Huestis
May 29, 2008·Biomedical Chromatography : BMC·E Gallardo, J A Queiroz
Apr 8, 2009·Rapid Communications in Mass Spectrometry : RCM·Susana Sadler SimõesMário João Dias
Jul 22, 2005·Journal of Mass Spectrometry : JMS
Aug 29, 2013·Drug Testing and Analysis·Dayong Lee, Marilyn A Huestis
Nov 13, 2013·Drug Testing and Analysis·Natalia BattistaMauro Maccarrone

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