Development and validation of a new transgenic hairless albino mouse as a mutational model for potential assessment of photocarcinogenicity

Mutation Research. Genetic Toxicology and Environmental Mutagenesis
Mugimane G ManjanathaMary D Boudreau

Abstract

Short-term phototoxicity testing is useful in selecting test agents for the longer and more expensive photocarcinogenesis safety tests; however, no validated short-term tests have been proven reliable in predicting the outcome of a photocarcinogenesis safety test. A transgenic, hairless, albino (THA) mouse model was developed that carries the gpt and red/gam [Spi(-)] genes from the gpt delta mouse background and the phenotypes from the SKH-1 mouse background to use as a short-term test in lieu of photocarcinogenesis safety tests. Validation of the THA mouse model was confirmed by exposing groups of male mice to sub-erythemal doses of ultraviolet B (UVB) irradiation for three consecutive days emitted from calibrated overhead, Kodacel-filtered fluorescent lamps and measuring the mutant frequencies (MFs) in the gpt and red/gam (Spi(-)) genes and types of mutations in the gpt gene. The doses or irradiation were monitored with broad-spectrum dosimeters that were calibrated to a NIST-traceable standard and cumulative CIE-weighted doses were 20.55 and 41.0mJ/cm(2) (effective). Mice were sacrificed 14 days after the final UVB exposure and MFs in both the gpt and red/gam genes were evaluated in the epidermis. The exposure of mice to UVB...Continue Reading

References

Nov 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·D E BrashJ Pontén
Jun 1, 1989·Photochemistry and Photobiology·D L Mitchell, R S Nairn
Nov 1, 1995·Molecular Carcinogenesis·T Basic-ZaninovicL Proitti-De Sactis
May 1, 1995·Journal of Photochemistry and Photobiology. B, Biology·L Daya-GrosjeanA Sarasin
Dec 22, 1994·Nature·A ZieglerD E Brash
Jun 1, 1994·Journal of Photochemistry and Photobiology. B, Biology·A A VinkL Roza
Jan 9, 1996·Proceedings of the National Academy of Sciences of the United States of America·E SageE A Drobetsky
Jun 21, 1996·International Journal of Cancer. Journal International Du Cancer·Z P RenJ Pontén
Aug 1, 1996·Nucleic Acids Research·A CalcagnileE Dogliotti
Jan 1, 1996·Environmental and Molecular Mutagenesis·T NohmiT Sofuni
Jan 1, 1997·Environmental and Molecular Mutagenesis·A F FrijhoffL Roza
May 6, 1998·Mutagenesis·J G de BoerB W Glickman
Jul 15, 1999·Photodermatology, Photoimmunology & Photomedicine·H MukhtarH N Ananthaswamy
Oct 26, 1999·Environmental and Molecular Mutagenesis·M HoriguchiT Nohmi
May 1, 2002·International Archives of Occupational and Environmental Health·M WeihrauchR Wrbitzky
Jan 29, 2003·Environmental and Molecular Mutagenesis·John A HeddleGeorge R Douglas
Apr 18, 2003·Frontiers in Bioscience : a Journal and Virtual Library·P Donald ForbesAntony R Young
Aug 30, 2003·Photodermatology, Photoimmunology & Photomedicine·Sleem F'guyerHasan Mukhtar
Mar 8, 2005·Mutation Research·Gerd P PfeiferAhmad Besaratinia
Feb 22, 2008·CA: a Cancer Journal for Clinicians·Ahmedin JemalMichael J Thun
Apr 25, 2008·Journal of the American Academy of Dermatology·Darrell S Rigel
Apr 25, 2008·Journal of the American Academy of Dermatology·Laurent Marrot, Jean-Roch Meunier
Jan 30, 2010·Actas dermo-sifiliográficas·P Aceituno-MaderaS Serrano-Ortega
Mar 17, 2010·Archives of Dermatology·Howard W RogersBrett M Coldiron
Oct 7, 2011·The Journal of Investigative Dermatology·Angela TewariAntony R Young
Nov 19, 2013·Journal of the American Academy of Dermatology·Rebecca JansenHenry W Lim
Jun 18, 2014·PloS One·Judy R ReesMargaret R Karagas
Oct 30, 2014·Photochemistry and Photobiology·Douglas E Brash

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacteriophage: Phage Therapy

Phage therapy uses bacterial viruses (bacteriophages) to treat bacterial infections and is widely being recognized as an alternative to antibiotics. Here is the latest research.

Carcinoma, Squamous Cell

Basal cell carcinoma is a form of malignant skin cancer found on the head and neck regions and has low rates of metastasis. Discover the latest research on basal cell carcinoma here.

Carcinoma, Basal Cell

Basal cell carcinoma is a form of malignant skin cancer found on the head and neck regions and has low rates of metastasis. Discover the latest research on basal cell carcinoma here.