Development and validation of a rapid multi-class method for the confirmation of fourteen prohibited medicinal additives in pig and poultry compound feed by liquid chromatography-tandem mass spectrometry

Journal of Pharmaceutical and Biomedical Analysis
Mark CronlyL Regan

Abstract

A confirmatory method has been developed to allow for the analysis of fourteen prohibited medicinal additives in pig and poultry compound feed. These compounds are prohibited for use as feed additives although some are still authorised for use in medicated feed. Feed samples are extracted by acetonitrile with addition of sodium sulfate. The extracts undergo a hexane wash to aid with sample purification. The extracts are then evaporated to dryness and reconstituted in initial mobile phase. The samples undergo an ultracentrifugation step prior to injection onto the LC-MS/MS system and are analysed in a run time of 26 min. The LC-MS/MS system is run in MRM mode with both positive and negative electrospray ionisation. The method was validated over three days and is capable of quantitatively analysing for metronidazole, dimetridazole, ronidazole, ipronidazole, chloramphenicol, sulfamethazine, dinitolimide, ethopabate, carbadox and clopidol. The method is also capable of qualitatively analysing for sulfadiazine, tylosin, virginiamycin and avilamycin. A level of 100 microg kg(-1) was used for validation purposes and the method is capable of analysing to this level for all the compounds. Validation criteria of trueness, precision, repe...Continue Reading

References

Nov 1, 1996·Journal of Pharmaceutical and Biomedical Analysis·H S TanW Cacini
Jul 15, 2000·Journal of Chromatography. a·G DusiA Baiguera
Oct 22, 2004·Journal of Pharmaceutical and Biomedical Analysis·C CivitarealeG Brambilla

❮ Previous
Next ❯

Citations

Apr 30, 2013·Journal of Chromatography. a·Kaklamanos GeorgeChristoph von Holst
Aug 22, 2013·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Gui-Jun ZhangLi-Min He
Apr 16, 2011·Journal of Agricultural and Food Chemistry·Sílvia BorràsJacinto Guiteras
Jun 25, 2013·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·S E McNameeC T Elliott
May 11, 2015·Journal of Pharmaceutical and Biomedical Analysis·Konrad PietrukTeresa Szprengier-Juszkiewicz
Jul 5, 2016·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·Christelle RobertNathalie Gillard
Jan 13, 2018·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·Ewelina PatyraKrzysztof Kwiatek
Jul 12, 2018·Journal of Mass Spectrometry : JMS·Xia ZhaoGuojun Dai
Dec 9, 2017·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·Ewelina PatyraKrzysztof Kwiatek
Sep 16, 2020·Analytical Methods : Advancing Methods and Applications·Jie LiuChuanlai Xu

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.