Development and validation of a real-time PCR for the detection and quantification of porcine circovirus type 2

Virusdisease
Ana Margarida HenriquesMiguel Fevereiro

Abstract

Porcine circovirus type 2 (PCV2) is a spherical and non-enveloped virus belonging to the genus Circovirus of the Circoviridae family with a single stranded circular DNA genome. This virus, already detected worldwide, has been associated to several diseases and was implicated as the etiological agent of a disease named postweaning multisystemic wasting syndrome. Several methods have been described for the detection of PCV2, being real-time PCR the most simple and reliable. As far as we know, all the real-time PCR systems described until now are based on ORF2 gene, that exhibit the highest variability. This paper reports the development and validation of a real-time PCR targeted to ORF1 and based on a TaqMan probe for the detection of porcine circovirus type 2 DNA in swine samples. Due to the lack of PCV1 samples, the ability of the test to discriminate between PCV1 and PCV2 positive samples was evaluated in silico. Estimations of 100% specificity and 100% sensitivity were obtained based on the qPCR results with panel of 81 swine samples (known PCV2-positive (n = 50); known PCV2-negative (n = 17); samples positive to other common swine viral pathogens (n = 13) and one sample from a BFDV-positive parrot (n = 1)). Intra- and inter-...Continue Reading

References

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Nov 24, 2006·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·L Mia KimDavid L Suarez
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Apr 15, 2016·Biomolecular Detection and Quantification·David SvecMikael Kubista

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Citations

Mar 4, 2020·Materials·Caroline R BassoJoão P Araújo Junior

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Datasets Mentioned

BETA
HQ831540
HQ831532
KJ680360
GU325763

Methods Mentioned

BETA
PCR
ELISA

Software Mentioned

GraphPad Prism

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