Development and Validation of a Real-Time PCR Assay for Rapid Detection of Candida auris from Surveillance Samples

Journal of Clinical Microbiology
L LeachS Chaturvedi

Abstract

Candida auris is an emerging multidrug-resistant yeast causing invasive health care-associated infection with high mortality worldwide. Rapid identification of C. auris is of primary importance for the implementation of public health measures to control the spread of infection. To achieve these goals, we developed and validated a TaqMan-based real-time PCR assay targeting the internal transcribed spacer 2 (ITS2) region of the ribosomal gene. The assay was highly specific, reproducible, and sensitive, with the detection limit of 1 C. auris CFU/PCR. The performance of the C. auris real-time PCR assay was evaluated by using 623 surveillance samples, including 365 patient swabs and 258 environmental sponges. Real-time PCR yielded positive results from 49 swab and 58 sponge samples, with 89% and 100% clinical sensitivity with regard to their respective culture-positive results. The real-time PCR also detected C. auris DNA from 1% and 12% of swab and sponge samples with culture-negative results, indicating the presence of dead or culture-impaired C. auris The real-time PCR yielded results within 4 h of sample processing, compared to 4 to 14 days for culture, reducing turnaround time significantly. The new real-time PCR assay allows f...Continue Reading

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Citations

Dec 6, 2018·Acta Clinica Belgica·Klaas DewaeleKatrien Lagrou
May 23, 2019·Journal of Occupational and Environmental Hygiene·Steven R TorresMagdia De Jesus
Feb 19, 2019·Mycoses·Shabir A Lone, Aijaz Ahmad
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Methods Mentioned

BETA
PCR
Assay

Software Mentioned

GraphPad Prism
MedCalc
PrimerQuest

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