Development and Validation of a Reversed-Phase Liquid Chromatography Method for the Simultaneous Determination of Indole-3-Acetic Acid, Indole-3-Pyruvic Acid, and Abscisic Acid in Barley (Hordeum vulgare L.).

Journal of Analytical Methods in Chemistry
Ilva NakurteNils Rostoks

Abstract

A simple, sensitive, precise, and specific reverse HPLC method was developed and validated for the determination of plant hormones in barley (Hordeum vulgare L.). The method includes extraction in aqueous organic solvent followed by solid-phase extraction, sample evaporation, and reversed-phase HPLC analysis in a general purpose UV-visible (abscisic acid (ABA)) and fluorescence detection (indole-3-acetic acid (IAA) and indole-3-pyruvic acid (IPA)), high-performance liquid chromatography system. The separation was carried out on Zorbax Eclipse XDB C8 column (150  ×  4.6  mm I.D) with a mobile phase composed of methanol and 1% acetic acid (60 : 40 v/v) in isocratic mode at a flow rate of 1 ml min(-1). The detection was monitored at 270 nm (ABA) and at 282 nm (Ex) and 360 nm (Em) (IAA, IPA). The developed method was validated in terms of accuracy, precision, linearity, limit of detection, limit of quantification, and robustness. The determined validation parameters are in the commonly acceptable ranges for that kind of analysis.

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Citations

Oct 29, 2013·Journal of Analytical Methods in Chemistry·Ibrahima Youm, Bi-Botti Celestin Youan
Dec 26, 2015·Analytica Chimica Acta·Sara PorfírioParastoo Azadi
May 12, 2015·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Guanhua XuanHuihui Liu
Apr 26, 2016·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Ruta MucenieceBaiba Jansone
Nov 5, 2016·Plant Molecular Biology·María Noelia Muñiz GarcíaDaniela Andrea Capiati
Sep 5, 2019·The Plant Journal : for Cell and Molecular Biology·Sharmila SinghAnanda K Sarkar
Jun 20, 2017·Frontiers in Plant Science·Agata Daszkowska-GolecIwona Szarejko
Aug 28, 2020·Frontiers in Plant Science·Anna CollinIwona Szarejko
Feb 14, 2021·Journal of Plant Physiology·Jacobo Pérez-PastranaNancy Santana-Buzzy

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