Development and validation of a sensitive method for the determination of ganciclovir in human plasma samples by reversed-phase high-performance liquid chromatography

Journal of Chromatography. B, Biomedical Sciences and Applications
M A CampaneroJ R Azanza

Abstract

A rapid, sensitive, specific liquid chromatographic method has been developed for the determination of therapeutic levels of ganciclovir in human plasma. Plasma (1 ml) and acyclovir (I.S.) were treated with 50% trichloroacetic acid. The supernatant was neutralized with 2 M NaOH and purified with chloroform. The aqueous phase (80 microl) was analyzed by a 3-microm Hypersil ODS C18 column with 0.04 M triethylamine-0.1 M sodium dihydrogen phosphate monohydrate as the mobile phase (1 ml/min) and ultraviolet detection at 254 nm. Calibration was linear from 50 to 10000 ng/ml. Intra- and inter-day C.V. did no exceed 6.65%. The detection limit was about 10 ng/ml.

References

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Citations

Oct 29, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Satoshi KishinoKatsumi Miyazaki
Oct 11, 2005·Journal of Perinatal Medicine·Caroline F Meine JansenAnton M van Loon
Nov 16, 2001·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·M TsuchieM Kai
Jul 17, 2012·Bioelectrochemistry·Nagaraj P ShettiSharanappa T Nandibewoor
May 24, 2006·Electrophoresis·Soundos Saleh, Georg Hempel
Oct 13, 2006·Journal of Pharmaceutical and Biomedical Analysis·Anders AsbergLeon Reubsaet
Jan 28, 2010·Transplant Infectious Disease : an Official Journal of the Transplantation Society·S LefeuvreE M Billaud
Jan 30, 2004·Therapeutic Drug Monitoring·Julie Cates ScottMary H H Ensom
Oct 27, 2015·Daru : Journal of Faculty of Pharmacy, Tehran University of Medical Sciences·Usha Ganganahalli KapanigowdaPrakash Rao Boggarapu
Jan 31, 2002·Journal of Chromatography. B, Biomedical Sciences and Applications·A LoregianE F De Palo

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