Development and validation of a simple capillary zone electrophoresis method for the analysis of kanamycin sulfate with UV detection after pre-capillary derivatization

Journal of Chromatography. a
E KaaleJos Hoogmartens

Abstract

Capillary zone electrophoresis was successfully applied to separate eight related substances of kanamycin and several minor unknowns from the main component. Strategies to enhance derivatization and selectivity and to optimize separation parameters involved the application of experimental designs. This chemometrical approach considers main effects as well as interactions of the influential parameters, thus conducting a more thorough investigation of the method than the common step-by-step approach. Central composite face centered designs established optimal separation conditions: 30 mM borax buffer, pH 10.0 containing 16.0% (v/v) methanol and optimal composition of derivatization reagent: 27 mg/ml 1,2-phthalic dicarboxaldehyde and 25 microl/ml mercaptoacetic acid in borate buffer, pH 10.4. The standard curves were linear over the concentration range of 0.007-1.01 mg/ml for the main component and 0.003-0.1 mg/ml for the related substances. The limit of quantitation was 0.14% (m/m) for the related substances and impurities (S/N= 10). The assay method was used to determine the composition of several commercial samples. Quantitative analysis indicates potential usefulness of capillary electrophoresis as an alternative to the assay ...Continue Reading

Citations

Jan 2, 2009·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Chang-Zhu YuWu-Er Gan
Sep 27, 2008·Electrophoresis·Abolghasem Jouyban, Ernst Kenndler
May 8, 2016·Talanta·Oliver Wahl, Ulrike Holzgrabe
Sep 22, 2005·Rapid Communications in Mass Spectrometry : RCM·Eliangiringa KaaleAnn Van Schepdael
Nov 3, 2006·Electrophoresis·Juan Manuel Serrano, Manuel Silva
Jul 20, 2020·Electrophoresis·Tiago Augusto DonegattiElisabete Alves Pereira

Related Concepts

Bacteriocidal Agents
Kantrex
Spectrophotometry, Ultraviolet
Electrophoresis, Capillary

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