Development and validation of a solid-phase extraction and high-performance liquid chromatographic assay for a novel fluorinated 2-nitroimidazole hypoxia probe (SR-4554) in Balb/c mouse plasma

Journal of Chromatography. B, Biomedical Applications
E O AboagyeM Tracy

Abstract

N-(2-Hydroxy-3,3,3-trifluoropropyl)-2-(2-nitro-1-imidazolyl) acetamide, a novel 2-nitroimidazole, is currently being developed as a non-invasive probe for tumour hypoxia. A sensitive (minimum quantifiable level = 25 ng/ml; C.V. = 6.01%) and selective assay has, therefore, been developed for the analysis of this compound in mouse plasma. The assay employed a solid-phase extraction followed by a rapid (10 min) HPLC analysis with UV-photodiode-array detection. No drug-related metabolites were observed in plasma when mice were treated with 180 mg/kg of the drug. The assay has proved to be suitable for studying the plasma pharmacokinetics of this fluorinated 2-nitroimidazole in mice.

References

Feb 1, 1978·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·I R FlockhartT R Marten
Sep 1, 1992·NMR in Biomedicine·P Vaupel

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Citations

Apr 2, 2013·Progress in Nuclear Magnetic Resonance Spectroscopy·Jian-Xin YuRalph P Mason
Sep 24, 2004·Molecular Imaging and Biology : MIB : the Official Publication of the Academy of Molecular Imaging·Serene S FooAndrew M Scott
Mar 20, 2014·British Journal of Pharmacology·K RamachandranL Stehno-Bittel

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