Development and Validation of a Universal One-Step RT-PCR Assay for Broad Detection of Duck Tembusu Virus.

Avian Diseases
Patchareeporn NinvilaiAunyaratana Thontiravong

Abstract

Duck Tembusu virus (DTMUV), a mosquito-borne flavivirus, has been identified as a causative agent of an emerging disease in ducks. Since its first report in 2010, several clusters of DTMUV have increasingly been identified and caused outbreaks in many Asian countries. This highlights the need for improved and novel broad detection assays in order to detect all circulating clusters of DTMUV. In this study, a universal one-step reverse-transcription PCR (RT-PCR) assay targeting a highly conserved region of the NS5 gene was developed and validated for broad detection of all DTMUV clusters. The newly developed universal RT-PCR assay could specifically detect all clusters of DTMUV without cross-reactions with common duck viruses and other related flaviviruses. The assay was able to detect DTMUV as low as a 0.001 50% embryo lethal dose/milliliter. The performance of the assay was evaluated by using experimental and field clinical samples. The assay could successfully detect DTMUV in all experimentally DTMUV-infected samples and gave a higher DTMUV detection rate (36%) than the previously reported envelope-specific RT-PCR assay (30%) in field clinical samples. All the positive samples were confirmed DTMUV-positive by DNA sequencing. I...Continue Reading

References

Dec 8, 2007·Virus Research·Mary RegatoJuan Cristina
Sep 22, 2010·Vector Borne and Zoonotic Diseases·Nicholas JohnsonAnthony R Fooks
Oct 18, 2011·Emerging Infectious Diseases·Zhenzhen CaoDabing Zhang
Oct 10, 2014·Avian Pathology : Journal of the W.V.P.A·Zalán Gábor HomonnayVilmos Palya
Mar 17, 2015·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Li DaiPan Tao
Apr 29, 2015·Antiviral Research·Siew Pheng LimPei-Yong Shi
Oct 8, 2015·Avian Pathology : Journal of the W.V.P.A·Yi TangYouxiang Diao
Nov 20, 2015·Emerging Infectious Diseases·Aunyaratana ThontiravongAlongkorn Amonsin
Jun 24, 2016·Journal of Medical Virology·Rama Adiga
Mar 4, 2017·The EMBO Journal·Wenqian DuanGeorge F Gao
Mar 28, 2017·Nature Communications·Boxiao WangJikui Song
Jun 20, 2017·BioMed Research International·Ariel Vina-RodriguezMartin Eiden
Mar 10, 2018·Transboundary and Emerging Diseases·P NinvilaiA Thontiravong
May 16, 2019·Transboundary and Emerging Diseases·Patchareeporn NinvilaiAunyaratana Thontiravong
Apr 11, 2020·Veterinary Microbiology·Patchareeporn NinvilaiAunyaratana Thontiravong

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