PMID: 8962662Mar 1, 1996Paper

Development and validation of an assay for measuring preimplantation factor (PIF) of embryonal origin

American Journal of Reproductive Immunology : AJRI
R G RoussevE R Barnea

Abstract

Tests to determine presence of embryos prior to implantation are needed. Sera from women after embryo transfer were tested for preimplantation factor (PIF) using the lymphocyte/platelet binding assay. Autorosettes were counted using blood type O+ donor lymphocytes and platelets incubated with blinded serum in the presence of antiCD2 antibody and rabbit complement. Human chorion gonadotropin (hCG) concentrations were determined 7 days later and compared with results of the lymphocyte/platelet assay. Implantation was confirmed by ultrasonographic evidence of presence of an intrauterine gestational sac. The roles of platelet activating factor (PAF) and chaperonin 10 in the observed phenomena were studied experimentally. Significantly more lymphocyte/platelet rosette formations were observed when sera from women who successfully implanted were compared to sera from women who failed to implant. Neither PAF nor chaparonin added to the tested sera controls influenced the percentage of lymphocyte/platelets rosettes. PIF is a likely candidate to be the next frontier of diagnosing the presence of viable preimplantation embryos in vivo.

References

Jan 1, 1992·Reproduction, Fertility, and Development·H MortonB E Rolfe
Apr 1, 1990·Clinical and Experimental Immunology·K A QuinnH Morton
Mar 1, 1990·American Journal of Reproductive Immunology : AJRI·F Nahhas, E Barnea
Sep 1, 1988·Journal of Reproduction and Fertility·N R Spinks, C O'Neill
Nov 1, 1989·Journal of Reproduction and Fertility·S Athanasas-PlatsisH Morton
Jan 20, 1989·Science·A O AbisogunA Tsafriri
Dec 1, 1988·The New England Journal of Medicine·I Hertz-Picciotto, S J Samuels
Jul 28, 1988·The New England Journal of Medicine·A J WilcoxB C Nisula
Nov 1, 1985·Journal of Reproduction and Fertility·C O'Neill
Jan 15, 1974·American Journal of Obstetrics and Gynecology·T M LinS Gall
Jan 1, 1995·American Journal of Reproductive Immunology : AJRI·R G RoussevC B Coulam
Oct 1, 1994·American Journal of Reproductive Immunology : AJRI·E R BarneaC B Coulam
Aug 1, 1995·American Journal of Reproductive Immunology : AJRI·C B CoulamE R Barnea

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Citations

Aug 17, 2010·American Journal of Obstetrics and Gynecology·Christina M DuzyjMichael J Paidas
May 20, 2000·Biotechnology & Genetic Engineering Reviews·A R CoatesP Mascagni
Jun 24, 2004·American Journal of Reproductive Immunology : AJRI·Eytan R Barnea
Oct 4, 2007·Annals of the New York Academy of Sciences·Eytan R Barnea

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