Development and validation of an ELISA for a biomarker of thyroid dysfunction, thyroid peroxidase autoantibodies (TPO-Ab), in dried blood spots.

Journal of Physiological Anthropology
Geeta N EickJ Josh Snodgrass

Abstract

The prevalence of allergic and autoimmune conditions has been steadily increasing in wealthy nations over the past century. One hypothesis put forward to explain this is the Old Friends Hypothesis, which posits that increased hygiene, urbanization, and lifestyle changes have reduced our exposure to parasites and microbes that we co-evolved with, resulting in immune dysregulation. However, research in traditionally living populations, who are exposed to greater parasite and pathogen loads such as those encountered during our evolution, is limited, in part due to a lack of minimally invasive, field-friendly biomarkers of autoimmune disorders. We therefore developed an ELISA to assess positivity for thyroid peroxidase autoantibody (TPO-Ab), an indicator of autoimmune thyroid disease, based on dried blood spot (DBS) samples. We used the Accubind anti-thyroid peroxidase test system to screen our validation samples comprising matched fingerprick DBS, venous DBS, and plasma samples from 182 adults. After confirming that we had TPO-Ab-positive individuals in our validation sample (n = 12), we developed an indirect ELISA to measure TPO-Ab levels from one 3-mm DBS punch. The sensitivity and specificity of our assay for DBS samples ranged...Continue Reading

References

May 5, 1981·Clinica Chimica Acta; International Journal of Clinical Chemistry·I R KupkeS Zeugner
Feb 12, 2002·The Journal of Clinical Endocrinology and Metabolism·Joseph G HollowellLewis E Braverman
Dec 23, 2004·The Journal of Laboratory and Clinical Medicine·Lindsay F HofmanEdwin W Naylor
Mar 1, 2005·Emergency Medicine Journal : EMJ·R BoydP Stuart
Dec 13, 2007·The Journal of Clinical Endocrinology and Metabolism·Anita KondrashovaHeikki Hyöty
Aug 15, 2009·Thyroid : Official Journal of the American Thyroid Association·Kanji KasagiYoichiro Izumi
Sep 1, 2006·Expert Review of Clinical Immunology·David Sinclair
Jul 7, 2011·American Journal of Human Biology : the Official Journal of the Human Biology Council·Tara J CeponVadim G Krivoshapkin
Oct 17, 2013·American Journal of Human Biology : the Official Journal of the Human Biology Council·Stephanie B LevyJames J Snodgrass
May 21, 2015·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Thomas StreckerStephan Becker
Feb 9, 2016·Thyroid : Official Journal of the American Thyroid Association·Rosaria M RuggeriSebastiano Gangemi
Sep 9, 2016·Hormone and Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et Métabolisme·M EhlersM Schott
Jan 11, 2017·Thyroid : Official Journal of the American Thyroid Association·Atieh AmouzegarFereidoun Azizi
Mar 25, 2017·Lancet·Layal ChakerRobin P Peeters
May 26, 2017·Frontiers in Immunology·Eleonore Fröhlich, Richard Wahl
Mar 24, 2018·Nature Reviews. Endocrinology·Peter N TaylorOnyebuchi E Okosieme

❮ Previous
Next ❯

Citations

Dec 9, 2020·American Journal of Human Biology : the Official Journal of the Human Biology Council·Tara J Cepon-Robins
Aug 11, 2021·American Journal of Human Biology : the Official Journal of the Human Biology Council·Alicia M DeLouizeJ Josh Snodgrass

❮ Previous
Next ❯

Methods Mentioned

BETA
ELISA
enzyme-linked immunoassay

Related Concepts

Related Feeds

Allergy and Asthma

Allergy and asthma are inflammatory disorders that are triggered by the activation of an allergen-specific regulatory t cell. These t cells become activated when allergens are recognized by allergen-presenting cells. Here is the latest research on allergy and asthma.

Autoimmune Diseases

Autoimmune diseases occur as a result of an attack by the immune system on the body’s own tissues resulting in damage and dysfunction. There are different types of autoimmune diseases, in which there is a complex and unknown interaction between genetics and the environment. Discover the latest research on autoimmune diseases here.