Development and validation of an ELISA kit (YF MAC-HD) to detect IgM to yellow fever virus

Journal of Virological Methods
Alison Jane BasileBarbara W Johnson

Abstract

Yellow fever virus (YFV) is endemic in tropical and sub-tropical regions of the world, with around 180,000 human infections a year occurring in Africa. Serologic testing is the chief laboratory diagnostic means of identifying an outbreak and to inform the decision to commence a vaccination campaign. The World Health Organization disseminates the reagents for YFV testing to African reference laboratories, and the US Centers for Disease Control and Prevention (CDC) is charged with producing and providing these reagents. The CDC M-antibody capture ELISA is a 2-day test, requiring titration of reagents when new lots are received, which leads to inconsistency in testing and wastage of material. Here we describe the development of a kit-based assay (YF MAC-HD) based upon the CDC method, that is completed in approximately 3.5h, with equivocal samples being reflexed to an overnight protocol. The kit exhibits >90% accuracy when compared to the 2-day test. The kits were designed for use with a minimum of equipment and are stored at 4°C, removing the need for freezing capacity. This kit is capable of tolerating temporary sub-optimal storage conditions which will ease shipping or power outage concerns, and a shelf life of >6 months was dem...Continue Reading

References

Jan 24, 2007·Vaccine·Ann W McMahonUNKNOWN Yellow Fever Working Group
May 31, 1937·The Journal of Experimental Medicine·M Theiler, H H Smith
Apr 23, 2010·Clinical and Vaccine Immunology : CVI·Kovi BessoffElizabeth Hunsperger
Nov 3, 2010·The American Journal of Tropical Medicine and Hygiene·Jaimie S RobinsonBarbara W Johnson
Jan 8, 2013·Journal of Clinical Laboratory Analysis·Harry E Prince, Cindy Yeh
Aug 8, 2014·Journal of Virological Methods·C H GoodmanB W Johnson

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Citations

Jul 20, 2018·Journal of Clinical Microbiology·Jesse J WaggonerBenjamin A Pinsky
Dec 13, 2019·Science Translational Medicine·Jody Hobson-PetersRoy A Hall
Jul 14, 2018·Emerging Microbes & Infections·Cristina DomingoChantal Reusken
Aug 24, 2018·Genes·Raphaëlle KlittingXavier de Lamballerie
Jun 5, 2021·PLoS Neglected Tropical Diseases·Christin H GoodmanAlison Jane Basile

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