Development and validation of an improved reversed-phase liquid chromatographic method combined with pulsed electrochemical detection for the analysis of netilmicin

Journal of Separation Science
Vicky ManyangaE Adams

Abstract

Netilmicin is one of the aminoglycoside antibiotics that lacks a strong UV absorbing chromophore. However, the application of pulsed electrochemical detection has been used successfully for the direct analysis of aminoglycoside antibiotics. This study describes an improved LC method combined with pulsed electrochemical detection for the analysis of netilmicin. Using a Zorbax SB C-18 column (250 mm x 4.6 mm id, 5 microm), isocratic elution was carried out with a mobile phase containing sodium sulfate (20 g/L), sodium octanesulfonate (0.3 g/L), THF (20 mL/L), and 0.2 M phosphate buffer pH 3.0 (50.0 mL/L). The robustness of the method was examined by means of an experimental design. The method proved to be sensitive, repeatable, linear, and robust. The method has also been used to analyze some commercial netilmicin samples.

Related Concepts

High Pressure Liquid Chromatography Procedure
Electrochemistry
Certomycin
Antibiotics, Aminoglycoside
Experimental Design
Netilmicin
Sodium sulfate
Sodium Butyrate
Cholecystectomy, Laparoscopic
Analysis

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