Development and validation of an inexpensive and efficient method for the extraction of Theileria orientalis DNA from blood

Veterinary Parasitology
D R BogemaC Jenkins

Abstract

Theileria orientalis is an emerging bovine pathogen in Australasia. PCR-based detection methods for this parasite are sensitive but relatively expensive, partly due to costs associated with DNA extraction. An inexpensive and efficient technique was developed for the extraction of T. orientalis DNA from blood based on hypotonic erythrocyte lysis and detergent-proteinase K treatment (DPK method). The DPK method compares favourably to a commercial extraction kit when paired with a T. orientalis multiplex qPCR.

References

Jan 11, 2007·The Journal of Veterinary Medical Science·Satoshi ZakimiChihiro Sugimoto
Feb 13, 2010·Australian Veterinary Journal·M M IzzoJ K House
Nov 16, 2010·Veterinary Parasitology·Natasha PapliAbdalla A Latif
Dec 29, 2010·The Journal of Veterinary Medical Science·Altangerel KhukhuuNaoaki Yokoyama
Sep 13, 2011·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·M Khyrul IslamRobin B Gasser
Mar 19, 2013·Parasitology International·Seung Won KangKi Yoon Chang
Aug 8, 2014·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Thillaiampalam SivakumarNaoaki Yokoyama
Mar 22, 2015·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·C JenkinsD R Bogema

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