Development and validation of an LC-ESI-MS/MS approach to determine a highly hydrophobic drug, norcantharidin palmitate, and apply to a preliminary pharmacokinetic study in rats

Biomedical Chromatography : BMC
Xiaolin LiuXing Tang

Abstract

In order to investigate the pharmacokinetics of norcantharidin palmitate (NCTD-PAL) in rats, we developed and validated an LC-ESI-MS/MS method. The NCTD-PAL and internal standard (triamcinoloneacetonide palmitate, TAP) were separated on a Phenomenex Kinetex®XB C18 column, and the mobile phase was composed of tetrahydrofuran (THF)-acetonitrile (20/80, v/v) and an aqueous phase containing 0.2% ammonium hydroxide at a flow rate of 0.3 mL/min. The ESI interface operated in positive mode was used to acquire the mass spectrometric data, and the transition ions were m/z 635.50 → 168.95 and 673.65 → 397.13 for NCTD-PAL and IS, respectively. The method had a linear range of 10-2000 ng/mL with a correlation coefficient of >0.99. The accuracy (RE, %) was within ±10.1%, and the intra- and inter-day precisions (RSD, %) were 10.9 and 13.8%, respectively. The extraction recovery of NCTD-PAL at different concentrations ranged from 89.3 to 102.0%. The validated approach was efficaciously applied to a pharmacokinetic study of NCTD-PAL in rats via intravenous injection. Based on these results obtained, this method is practical and suitable for a wide range of applications.

References

Jun 1, 1995·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·X H LiuJ L Misset
Dec 10, 1999·Journal of Mass Spectrometry : JMS·Z KeleP T Szabo
Sep 29, 2005·Drug Delivery·Li ZhangZhi-Rong Zhang
Oct 20, 2006·Journal of Controlled Release : Official Journal of the Controlled Release Society·Xiaohua LiuLai Wah Chan
Dec 18, 2007·International Journal of Pharmaceutics·Shaobing Wang, Shengrong Guo
Aug 25, 2009·Nanomedicine : Nanotechnology, Biology, and Medicine·Qin WangQiang Zhang
Aug 5, 2014·International Journal of Pharmaceutics·Jinlong MaCuifang Cai
Jul 9, 2016·Journal of Pharmaceutical and Biomedical Analysis·Jahidur Rashid, Fakhrul Ahsan
Dec 7, 2016·Journal of Pharmaceutical and Biomedical Analysis·Xiabin ChenFang Zheng

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